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Q: The doctor says Urine culture result no grpwth after 3 days incubation?
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Why bacteria grows faster ion open air than in refrigerator?

The incubation time is important because however long your culture is kept in incubation affects how many colonies you will have at the end. A short incubation time will result in minor bacterial growth, whereas a longer incubation will result in more colonies, as you're giving the bacteria more time to grow. Be careful, if you leave your culture in incubation for too long, the bacteria will begin to use up all of the agar and start competing for food resources. If left even longer, this will consequently result in the death of your culture.


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Why is it acceptable to record a positive result before the suggested incubation timne is completed but it is not acceptable to record a negative result early when doing a urea hydrolysis test?

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1. It is less likely to get a false positive result. Usually the test can detect the pure organism right away. It is therefore acceptable to record positive test before suggested incubation time is completed. A negative result should not be recorded early because sometimes it takes longer for bacteria/organism to hydrolyze the material.


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