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Why is it important to incubate your culture for more than 5 days when performing the indole test?
Wiki User
∙ 10y ago
If incubation surpasses 5 days, the bacteria may degrade indole to the point that a FALSE negative would result.
Wiki User
∙ 10y ago
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Explain the chemical mechanism for detecting indole in a bacterial culture?
Indole is extracted from the medium and into the reagent layer (Kovac's) by the acidified butyl alcohol component and forms a complex with the p-dimethylaminobenzaldehyde, yielding the cherry red color seen when the culture is indole positive.
How do you get proteus Mirabilis?
By performing indole test and examining change in phenyl alanine deaminase agar.
What is an indole alkaloid?
class of alkaloids containing a structural moiety of indole.
What does the S.I.M. test for?
Sulfur Indole Motility test
What test will differentiate between Proteus vulgaris and Citrobacter freundii?
Indole test,Proteus vulgaris is be indole positive and Citrobacter freundii will be indole negative
What are the indole test principles?
this test is done to determine if bacteria can breakdown the amino acid tryptophan into indole
Why determine motility and h2s before adding kovac's reagent?
You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.
Indole test in vibro cholera?
The indole positive test is used to test the absence or presence of the vibro cholera.
What is the substrate for the Indole test?
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to split indole from the amino acid tryptophan. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as "tryptophanase."
What is the chemical formula of Indole?
C8H7N
What Enzyme changes tryptophane to indole?
tryptophanase
Is staphylococcus epidermidis indole positive?
S.aureus is a positive gram. Indole test is a part the biochemistry test to determinate a bacteria negative gram. indole test used to determine S.aureus, the result is negative. That is improve which S.aureus haven't trytophanase enzym (please see indole test principle)bcb
