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it is used in gel electrophoresis.....for the separation of DNA fragments
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
Gel electrophoresis is an analytical method used to separate DNA, RNA or proteins based on size
Just make sure that you're using Cross-Linked Polyacrylamide Crystals; otherwise, it can contain traces of Acrylamide monomer and that stuff is a neurotoxin. What I use my crystals for is combining with water to create a gel. I use pieces of this gel as a safe water source for worms and crickets that I raise as food source for my pet gecko. Rather than providing water, the gel water source ensures that the insects don't accidentally drown themselves. By purchasing the cross-linked form of the crystals, when my gecko eats the insects I have raised, I don't have to worry about trace amounts of Acrylamide accumulating in his system, which could result in serious health problems later on.
Polyacrylamide gel electrophoresis (PAGE) is used to separate large biomolecules, such as RNA, DNA and proteins, by their size, shape and charge. Proteins can be separated based on their size alone if the sample is treated with a denaturing agent first, such as sodium dodecyl sulfate (SDS). Nucleic acid samples can be separated on size alone through the use of an agarose gel.
a Polyacrylamide gel
it is used in gel electrophoresis.....for the separation of DNA fragments
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
its starch ,agarose and polyacrylamide by mkhaliphi dlamini
The migration of DNA/Protein on the gel (agarose/polyacrylamide) by the influence of electric charge is called gel electrophoresis. It is used to resolve the biomolecules according to their size(mainly) and shape(for proteins)
total amount of monomer present (%T) and the amount of cross- linker (%C).
Acrylamide forms polymers (polyacrylamide) that acts as a cross-linked matrix to "catch" the proteins as they run across the gel to the positive end. The polyacrylamide gel is composed of different sizes of pores that allows for separation based on size. As a result, small proteins travel faster and bigger proteins travel slower.
I am a researcher and I worked on population genetics of Deers by Microsatellite in IRAN. I have prepared many gel polyacrylamide from primers. I need calculat the Bands on gel for using in POP GENE software.
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
Polyacrylamide is a polymer that absorbs liquid.
If you meant "protein gel electrophoresis" (considering the image on this page) is a very powerful technique and widely used to separate proteins according to their mass, molecular weight and charge. The support most used for this technique is the polyacrylamide.
The chemical formula for polyacrylamide is (C3H5NO)n