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Well child, you could increase the density of the gel and that would allow for the smaller of the two fragments to move farther, but keep the larger one of the two from moving just as far.

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Q: Two small restriction fragments of nearly the same?
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Who has exactly the same DNA restriction fragments?

two identical DNA fragments will have identical restriction fragments. Also, genetically identical twins will have identical restriction fragments


Does a restriction enzyme generate the same size fragments in genomic DNA of different species?

No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.


Two small restriction fragments of nearly the same base pair size appear as a single band even when the sample is run to the very end of the gel What could be done to resolve the fragments?

Increase the density of the gel. This will slow down the denser fragment while speeding up the less dense fragment. The down side is that the denser fragments near the beginning of the gel near the DNA well will be seen as a single band.


Why might different alleles of the same gene produce different fragments when treated with the same restriction enzyme?

Well..... It is quite simple. if you take the square of the number of alleles and then times that by the smaller of the height of the triangle you should get your answer.


The rate at which large DNA fragments move through the electrophoretic gel is?

the answer to this question is that, its greater than the rate at which small DNA fragments move through the same apparatus


How do you use electrophoresis to determine paternity of a baby?

Children receive half of their genetic material from each parent. There are specific sites on DNA, known as restriction sites, that are recognized by restriction enzymes. These are used to determine paternity. Samples of DNA from the mother, father and child are taken. They are all digested ('cut') by the same restriction enzymes. These DNA fragments are then separated by gel electrophoresis (which separates fragments based on size). The bands of the child are compared to the mother and father's. If the band is not the same as the mother's, it must have come from the father. If these do not match up, then the sample was not taken from the biological father.


Why did you cut both segments of DNA with the same restriction enzyme?

You use the same enzyme inn order to get the same restriction and binding sites.


How is transformation used to construct recombinant DNA molecules?

DNA fragments have to be cut precisely and then joined with the other fragment by using a ligase.To begin with, the DNA fragments to be joined should be cut with the same restriction enzyme or should have compatibility to join. Restriction enzymes make cuts at specific recognition sites. Hence restriction digestion with same enzyme for two different DNA fragments yields identical ends.Two DNA fragments with protruding ends complementary to each other are joined using a Ligase and blunt ended DNA fragments are joined by means of adding additional complementary sequences at their ends with the help of linkers, adapters or by using a terminal transferase for Homopolymer Tailing.


What name is given to a region of DNA that varies from person to person?

Restriction fragments are the region of DNA that varies from person to person. This is the key for DNA testing as many parts of the DNA string are the same for all mankind.


What does the formation of recombinant DNA result from?

Recombinant DNATo to make recombinant DNA or plasmids, the two different samples of DNA need to be cut up by the same restriction enzyme. Restriction enzymes cut DNA at specific sequences (restriction sites) and is usually a staggered cut. For example, say you had the following sequence of DNA (both strands): 5' GAATTC 3'3' CTTAAG 5'Say the restriction enzyme used will cut a strand between a guanine and adenine on one strand and an adenine and guanine one the other strand. For the given DNA, there would be cuts where the bars are:5' G|AATTC 3'3' CTTAA|G 5'Then the strands would separate:5' G--------AATTC 3'3' CTTAA--------G 5'Because the cuts are staggered, hydrogen bonds are left open. The ends of the restriction fragments are called "sticky ends" because of their ability to bond to other fragments. Remember that both sets of DNA are cut with the same restriction enzyme. Therefore, the sticky ends of the restriction fragments are complementary to each other. Then you're able to take one fragment of one DNA sample and insert it into the other DNA sample, which are bound together by hydrogen bonds. DNA ligase is then added to seal the ends together.


What is Example of restriction enzyme?

A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'


Two different restriction enzymes that recognize the same restriction site known as?

Two different DNA sequences