DNA fragments have to be cut precisely and then joined with the other fragment by using a ligase.
To begin with, the DNA fragments to be joined should be cut with the same restriction enzyme or should have compatibility to join. Restriction enzymes make cuts at specific recognition sites. Hence restriction digestion with same enzyme for two different DNA fragments yields identical ends.
Two DNA fragments with protruding ends complementary to each other are joined using a Ligase and blunt ended DNA fragments are joined by means of adding additional complementary sequences at their ends with the help of linkers, adapters or by using a terminal transferase for Homopolymer Tailing.
Transformation involves making cell competant so that they can uptake DNA molecules easily. So basically after carrying out the Transformation procedure you may get the cells which are transformed as well as non-transformed. Thus we now need to select the transformed one which is usually done by using Blue-White screening which results only in the selection of transformed cells.
Well recombinant DNA are DNA that have DNA parts from other organisms. The DNA from the organisms, at least two, are then combined using modern engineering techniques. -Hope I answered your question
it's used to insert genes into a plant.
When DNA contains parts from two or more organisms it is recombined. Recombinant DNA is often used in genetic engineering. A natural process of DNA recombination is called sexual reproduction.
I know of one commonly used bacteria: E. Coli
"trojan horse" recombinant vaccine
restriction enzymes
No
yes
Well recombinant DNA are DNA that have DNA parts from other organisms. The DNA from the organisms, at least two, are then combined using modern engineering techniques. -Hope I answered your question
write 10 recombinant proteins which are used in medical practice where they are used as therapeutics?
photosynthesis
How can recombinant DNA technology be used to combat HIV infections?
Recombinant DNA technology
Recombinant DNA technology PCR
it's used to insert genes into a plant.
ipad
penus