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Complementary base pairing.

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Pearline Blick

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3y ago

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What allows a DNA probe to find a single-stranded target gene?

Diploid cells


What property of DNA makes it possible for a probe to find a single-stranded DNA target gene?

a DNA molecule has two paired strands. ~


Why does a probe hybridize to a target gene but not to any other unrelated gene?

Complementary base pairing occurs only between the probe and the target gene.


Which explains how a probe can find a single-standard target DNA gene?

The probe is the second strand of DNA that forms double-stranded DNA with the target gene.


What property of DNA makes hybridization between a labeled probe and a target gene possible?

The complementary base pairing between DNA strands enables hybridization between a labeled probe and a target gene. The hydrogen bonding between adenine-thymine and guanine-cytosine base pairs allows the probe to specifically bind to its complementary sequence in the target gene, facilitating detection.


Why a probe will hybridize to a target gene?

A probe will hybridize to a target gene due to complementary base pairing between the nucleotides of the probe and the target sequence. This specificity allows the probe, often labeled for detection, to bind to its complementary region on the target gene under appropriate conditions, such as temperature and salt concentration. The hybridization process is driven by the stability of the double-stranded DNA formed, which is influenced by factors like sequence complementarity and the presence of chemical modifications. This property is widely utilized in techniques such as PCR, microarray analysis, and in situ hybridization for gene detection and analysis.


What is a DNA probe?

A Gene Probe is a term used to label several techniques used to identify a Gene Loci. a gene probe is often a piece of genetic material, that mirrors the target gene, and is also 'tagged' with dye so it can be identified.


Explain why a probe will hybridize to a target gene but not to any unrelated genes?

We design probes to be specific so that they will hybridize only to the target gene but not to random, unrelated genes. A probe hybridizes to a sequences to which it is complementary to. Consequently, the longer the probe is, the more specific it will be.


What explains why a probe will hybridized to a target gene but not to any unrelated genes?

We design probes to be specific so that they will hybridize only to the target gene but not to random, unrelated genes. A probe hybridizes to a sequences to which it is complementary to. Consequently, the longer the probe is, the more specific it will be.


Which feature of DNA enables a single-stranded DNA probe to hybridize with a single-stranded target gene?

The complementary base pairing between adenine (A) and thymine (T), and cytosine (C) and guanine (G) allows a single-stranded DNA probe to hybridize with a single-stranded target gene. This specificity is key for detecting and identifying specific sequences in the target gene through base pairing interactions.


How can a probe find a single-stranded target DNA gene apex?

A probe can find a single-stranded target DNA gene apex by hybridizing to its complementary sequence. This is often achieved through techniques like fluorescence in situ hybridization (FISH) or polymerase chain reaction (PCR), where the probe is labeled with a fluorescent tag or a reporter molecule. The specificity of the probe ensures that it binds only to the target sequence, allowing researchers to detect and analyze the gene of interest among other genetic material.


What is a gene probe used for?

Eating a watermelon.