Okazaki fragments.
A DNA LibraryA collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids is called a DNA library. RNA can manufacture DNA via the action of reverse transcriptase.
DNA polymerase I removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments in prokaryotes.
The lagging strand of DNA is replicated using a process called Okazaki fragments. These are short DNA fragments synthesized in the 5' to 3' direction by DNA polymerase, and are subsequently joined together by DNA ligase to form a continuous strand.
A DNA LibraryA collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids is called a DNA library. RNA can manufacture DNA via the action of reverse transcriptase.
Adenine bonds with thymine in DNA and uracil in RNA.
The process that involves the transcribing of genetic information from DNA to RNA is called Transcription.
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
The two main substances that make up a virus are nucleic acid (DNA or RNA) and protein. When these substances are broken into smaller fragments, the nucleic acid fragments are called nucleotides, and the protein fragments are called amino acids.
DNA replication
c-DNA library is a combination of cloned c-DNA(complementary DNA)fragments inserted into a collection of host cells which together constitute some portion of transcriptome(it is a set of all RNA molecules including m-RNA,r-RNA,t-RNA and other non-coding RNA produced in one or a population of cells) of an organism.c-DNA is produced from fully transcribed m-RNA found in the nucleus and therefore contains only the expressed genes of an organism.
RNA primase is used to synthesize short RNA primers that are needed for DNA replication by DNA polymerase. This RNA primer can be easily replaced by DNA once DNA polymerase starts synthesizing the new DNA strand. This is different from DNA primase which synthesizes RNA primers during the synthesis of Okazaki fragments on the lagging strand during DNA replication in prokaryotes and eukaryotes.
The lagging strand is synthesized in fragments called Okazaki fragments. These fragments are short sequences of DNA synthesized in the opposite direction of the replication fork. Once the DNA polymerase reaches the next RNA primer, a new fragment begins.