DNA Polymerase I
In DNA replication, the two DNA strands acting as templates need to be synthesized simultaneously. DNA polymerase is an enzyme which can synthesize the DNA only in 5' to 3' direction.the two template strands are anti-parallel to each other and their complementary strands are synthesized in different direction. In one of the strand DNA is synthesized continuously by adding nucleotides at 3'-OH end. this is referred as 'leading strand' synthesis. the other strand to be synthesized is replicated in short fragments referred 'Okazaki fragments' named after their discoverer Reiji Okazaki.
The fragments making up the noncontinuous strand in DNA replication are called Okazaki fragments. These are short DNA fragments that are synthesized discontinuously on the lagging strand during DNA replication.
Okazaki fragments are the small DNA fragments synthesized on the lagging strand during DNA replication. They are later joined together by DNA ligase to form a continuous strand.
An Okazaki fragment is a short, newly synthesized DNA fragment that is formed on the lagging strand during DNA replication. It is composed of a short RNA primer at the 5' end and DNA nucleotides that extend toward the replication fork.
Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction) never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction. The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'. Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase. Here I attache a link to a flash video that will make u understand better! http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf
DNA ligase is the enzyme responsible for connecting the Okazaki fragments on the lagging strand during DNA replication. It catalyzes the formation of phosphodiester bonds to join the individual nucleotides together.
During DNA replication Okazaki fragments are joined together by DNA polymerase. Remember that Okazaki fragments start with an RNA primer so RNAse H is need to remove the primer follwed by DNA plymerase to add nucleotides and finally DNA ligase to seal the single strand nick.
Okazaki fragments.
okazaki fragments are new pieces of Dna On the lagging strand
In DNA replication, the two DNA strands acting as templates need to be synthesized simultaneously. DNA polymerase is an enzyme which can synthesize the DNA only in 5' to 3' direction.the two template strands are anti-parallel to each other and their complementary strands are synthesized in different direction. In one of the strand DNA is synthesized continuously by adding nucleotides at 3'-OH end. this is referred as 'leading strand' synthesis. the other strand to be synthesized is replicated in short fragments referred 'Okazaki fragments' named after their discoverer Reiji Okazaki.
Okazaki fragments
Okazaki fragments
The fragments making up the noncontinuous strand in DNA replication are called Okazaki fragments. These are short DNA fragments that are synthesized discontinuously on the lagging strand during DNA replication.
Okazaki fragments are the small DNA fragments synthesized on the lagging strand during DNA replication. They are later joined together by DNA ligase to form a continuous strand.
An Okazaki fragment is a short, newly synthesized DNA fragment that is formed on the lagging strand during DNA replication. It is composed of a short RNA primer at the 5' end and DNA nucleotides that extend toward the replication fork.
It joins Okazaki fragments
okazaki fregments