5' DNA to 3'
Bipin
Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction) never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction. The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'. Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase. Here I attache a link to a flash video that will make u understand better! http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf
DNA polymerase I removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments in prokaryotes.
Okazaki fragments are typically around 100-200 base pairs long in prokaryotes and around 1000-2000 base pairs long in eukaryotes.
Okazaki fragments are the small DNA fragments synthesized on the lagging strand during DNA replication. They are later joined together by DNA ligase to form a continuous strand.
The lagging strand will have the Okazaki fragments. These short fragments are created as the DNA replication machinery synthesizes the new DNA strand discontinuously in the 5'-3' direction away from the replication fork.
They are formed on the lagging strand of DNA.
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Reiji and Tsuneko Okazaki, along with colleagues, discovered short DNA fragments called Okazaki fragments that are synthesized discontinuously during DNA replication on the lagging strand. Their work helped to elucidate the process of DNA replication and how it occurs on both the leading and lagging strands, leading to the development of the Okazaki fragment model for DNA replication.
Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction) never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction. The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'. Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase. Here I attache a link to a flash video that will make u understand better! http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf
I'm happy to help! Please provide the item you would like me to check for a fragment.
Bob Okazaki's birth name is Iwao Robert Okazaki.
Oliver Okazaki's birth name is Oliver Kenji Okazaki.
ssb protein bind to the lagging strand as leading strand is invovled in dna replication and lagging strand is invovled in okazaki fragment formation
You can find reliable information about travel arrangements on the following webpage: http://traveltips.usatoday.com/travel-arrangements-13279.html
Miles Okazaki was born in 1974.
Tomiko Okazaki was born in 1944.
Reiji Okazaki died in 1975.