50 base pairs
Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction) never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction. The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'. Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase. Here I attache a link to a flash video that will make u understand better! http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf
The leading strand is synthesized continuously in the 5' to 3' direction, making replication faster and more efficient. The lagging strand is synthesized discontinuously in short fragments called Okazaki fragments, which are later joined together by DNA ligase. This process of replication is slower and requires additional steps compared to the leading strand.
The fragment of the DNA that is the longest is the one that is the slowest to get to the bottom of the gel in the body. This is because longer DNA are simply the largest base pairs that are digested in the restriction enzymes which make them slower then the shorter ones.
A human cell typically contains about 6.4 billion base pairs of DNA, which is spread across 46 chromosomes (23 pairs). Each chromosome carries a different segment of DNA, resulting in a total of approximately 3.2 billion base pairs in a human cell.
Since each amino acid is coded for by a combination of three nucleotide bases (a codon), the number of nucleotides in the gene sequence would be 3300 * 3 = 9900 nucleotide base pairs long.
Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction) never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction. The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'. Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase. Here I attache a link to a flash video that will make u understand better! http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf
No, DNA is not always six base pairs long. The length of DNA can vary and is determined by the number of nucleotide base pairs present in the DNA molecule. The human genome, for example, consists of about 3 billion base pairs.
The leading strand is synthesized continuously in the 5' to 3' direction, making replication faster and more efficient. The lagging strand is synthesized discontinuously in short fragments called Okazaki fragments, which are later joined together by DNA ligase. This process of replication is slower and requires additional steps compared to the leading strand.
DNA strands can vary in length based on the specific sequence of nucleotides needed for a particular gene. The length of a DNA strand is determined by the number of base pairs required to encode the necessary genetic information to produce a functional product, such as a protein or RNA molecule. Different genes have different lengths of DNA sequences, resulting in varying numbers of base pairs in the DNA strand.
Both strands are labeled, but Okazaki's trick was to use T4 bacteriophages that had a mutated ung gene- these viruses didn't produce ligase. Without ligase, the fragments kept accumulating.For example: a 10 second pulse would show lots of fragments in normal and mutant T4 phages (including lagging and short leading strands), whereas a 60 second pulse would show mostly long DNA pieces in normal phages but lots and lots of fragments as well as some longer pieces of DNA in the mutant phages.
The fragment of the DNA that is the longest is the one that is the slowest to get to the bottom of the gel in the body. This is because longer DNA are simply the largest base pairs that are digested in the restriction enzymes which make them slower then the shorter ones.
There are several different strains of E. coli, each having about five million (5,000,000) base pairs. For example, uropathogenic E. coli (the one commonly associated with urinary tract infections) has about 5,231,428 base pairs, while E. coli K-12 has 4,639,221. The number of base pairs an organism has in its genes is commonly referred to as genome size. A web search for "genome size E. coli" is how I found these numbers.
A human cell typically contains about 6.4 billion base pairs of DNA, which is spread across 46 chromosomes (23 pairs). Each chromosome carries a different segment of DNA, resulting in a total of approximately 3.2 billion base pairs in a human cell.
Both genes and genomes come in a variety of sizes. About 1,000 base pairs would be enough DNA to encode most proteins. But introns-"extra" or "nonsense" sequences inside genes-make many genes longer than that. Human genes are commonly around 27,000 base pairs long, and some are up to 2 million base pairs. Very simple organisms tend to have relatively small genomes. The smallest genomes, belonging to primitive, single-celled organisms, contain just over half a million base pairs of DNA. But among multicellular species, the size of the genome does not correlate well with the complexity of the organism. The human genome contains 3 billion base pairs of DNA, about the same amount as frogs and sharks. But other genomes are much larger. A newt genome has about 15 billion base pairs of DNA, and a lily genome has almost 100 billion.
Genes can vary in size, but the largest known gene in humans is the dystrophin gene, which is about 2.4 million base pairs long.
Since each amino acid is coded for by a combination of three nucleotide bases (a codon), the number of nucleotides in the gene sequence would be 3300 * 3 = 9900 nucleotide base pairs long.
it devolves into sand