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Okazaki fragments are only used on the Lagging strand (the one going on 5' to 3' direction)
never on the leading one. In fact all that the leading strand uses is the helicase to unwind DNA and DNA polimerase III to form the complementary strand allways in 3' to 5' (the leading strand) direction.
The big problem of the laggind strand and the reason that Okazaki fragments exist and all other complementary DNAs (polimerace I, ligase, SSB, primace) is that it runs from 3' to 5'.
Now what Okazaki fragments are, is temporary pieces of complementary DNA (iniciated by a primer)that are not joined together, but that later on before it goes back to the helix form will be joined togather by DNA ligase.
Here I attache a link to a flash video that will make u understand better!
http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.swf
What else can I help you with?
An okazaki fragment has which of the following arrangements?
An Okazaki fragment is a short, newly synthesized DNA fragment that is formed on the lagging strand during DNA replication. It is composed of a short RNA primer at the 5' end and DNA nucleotides that extend toward the replication fork.
How long are the base pairs of Okazaki fragments?
Okazaki fragments are typically around 100-200 base pairs long in prokaryotes and around 1000-2000 base pairs long in eukaryotes.
What are the small segments synthesized in the lagging strand of DNA?
Okazaki fragments are the small DNA fragments synthesized on the lagging strand during DNA replication. They are later joined together by DNA ligase to form a continuous strand.
Which strand (leading or lagging) will have the okazaki fragments?
The lagging strand will have the Okazaki fragments. These short fragments are created as the DNA replication machinery synthesizes the new DNA strand discontinuously in the 5'-3' direction away from the replication fork.
How should you contruct YAC for a cloning 50kb size fragment?
To clone a 50kb fragment using Yeast Artificial Chromosomes (YAC), you should start by isolating the DNA fragment and then inserting it into a YAC vector using homologous recombination in yeast cells. This process involves transforming the yeast cells with the modified YAC vector containing the 50kb fragment, selecting for successful transformations, and confirming the presence of the fragment in the YAC. Additionally, you will need to maintain the YAC in yeast cells and perform quality control checks to ensure stability and integrity of the cloned fragment.
Which of the following statements about okazaki fragment in e coli is true?
They are formed on the lagging strand of DNA.
An okazaki fragment has which of the following arrangements?
An Okazaki fragment is a short, newly synthesized DNA fragment that is formed on the lagging strand during DNA replication. It is composed of a short RNA primer at the 5' end and DNA nucleotides that extend toward the replication fork.
What was the contribution of reiji and tuneko okazaki and colleagues with regard to these replication models?
Reiji and Tsuneko Okazaki, along with colleagues, discovered short DNA fragments called Okazaki fragments that are synthesized discontinuously during DNA replication on the lagging strand. Their work helped to elucidate the process of DNA replication and how it occurs on both the leading and lagging strands, leading to the development of the Okazaki fragment model for DNA replication.
What is the birth name of Bob Okazaki?
Bob Okazaki's birth name is Iwao Robert Okazaki.
What is the birth name of Oliver Okazaki?
Oliver Okazaki's birth name is Oliver Kenji Okazaki.
Where does ssb protein bind on lagging strand or on leading strand?
ssb protein bind to the lagging strand as leading strand is invovled in dna replication and lagging strand is invovled in okazaki fragment formation
When was Hisahiko Okazaki born?
Hisahiko Okazaki was born in 1930.
When was Miles Okazaki born?
Miles Okazaki was born in 1974.
When was Kenjiro Okazaki born?
Kenjiro Okazaki was born in 1955.
When was Tomiko Okazaki born?
Tomiko Okazaki was born in 1944.
When did Reiji Okazaki die?
Reiji Okazaki died in 1975.
When was Reiji Okazaki born?
Reiji Okazaki was born in 1930.
