What are Gram positive vs gram Negative?

Is aspergillus gram positive?

Traditionally, Gram positive and Gram negative are terms used to describe bacteria (Eubacteria more specifically). Aspergillus species are Fungi, they belong in an entirely different kingdom of taxonomy from bacteria (Fungi vs. Monera). Therefore they are neither Gram positive or Gram negative per se.

Is the gram stain of significant importance in identifying hemolysis?

No, the Gram stain is primarily used to differentiate bacteria based on their cell wall structure (Gram-positive vs. Gram-negative). Hemolysis is a test used to determine the ability of bacteria to lyse red blood cells, which can provide information on the pathogenicity of the organism.

Can you gram stain a cell without a cell wall?

No, Gram staining primarily distinguishes bacteria based on their cell wall composition (Gram-positive vs Gram-negative). Cells without a cell wall, such as animal cells, cannot be Gram stained due to the absence of the target structure for the stain to bind to.

What can result in a gram-positive organism staining gram-negative?

The following characteristics are generally present in a Gram-positive bacterium.cytoplasmic lipid membrane teichoic acids and lipoids are present, forming lipoteichoic acids which serve to act as chelating agents, and also for certain types of adherence.thick peptidoglycan layercapsule polysaccharides (only in some species) if present, it contains two rings for support as opposed to four in Gram-negative bacteria because Gram-positive bacteria have only one membrane layer.flagellum (only in some species)Although there is only difference in gram positive and negative is of cell wall as :The Gram positive cell wallThe Gram positive cell wall is characterised by the presence of a very thick peptidoglycan layer, which is responsible for the retention of the crystal violet dyes during the Gram staining procedure. It is found exclusively in organisms belonging to the Actinobacteria (or high %G+C Gram positive organisms) and the Firmicutes (or low %G+C Gram positive organisms). Bacteria within the Deinococcus-Thermus group may also exhibit Gram positive staining behaviour but contain some cell wall structures typical of Gram negative organisms. Imbedded in the Gram positive cell wall are polyalcohols called teichoic acids, some of which are lipid-linked to form lipoteichoic acids. Because lipoteichoic acids are covalently linked to lipids within the cytoplasmic membrane they are responsible for linking the peptidoglycan to the cytoplasmic membrane. Teichoic acids give the Gram positive cell wall an overall negative charge due to the presence of phosphodiester bonds between teichoic acid monomers.The Gram negative cell wallUnlike the Gram positive cell wall, the Gram negative cell wall contains a thin peptidoglycan layer adjacent to the cytoplasmic membrane, which is responsible for the cell wall's inability to retain the crystal violet stain upon decolourisation with ethanol during Gram staining. In addition to the peptidoglycan layer the Gram negative cell wall also contains an additional outer membrane composed by phospholipids and lipopolysaccharides which face into the external environment. The highly charged nature of lipopolysaccharides confer an overall negative charge to the Gram negative cell wall. The chemical structure of the outer membrane lipopolysaccharides is often unique to specific bacterial strains (i.e. sub-species) and is responsible for many of the antigenic properties of these strains.As a phospholipid bilayer, the lipid portion of the outer membrane is largely impermeable to all charged molecules. However, channels called porins are present in the outer membrane that allow for passive transport of many ions, sugars and amino acids across the outer membrane. These molecules are therefore present in the periplasm, the region between the plasma membrane and outer membrane. The periplasm contains the peptidoglycan layer and many proteins responsible for substrate binding or hydrolysis and reception of extracellular signals. The periplasm is thought to exist as a gel-like state rather than a liquid due to the high concentration of proteins and peptidoglycan found within it. Because of its location between the cytoplasmic and outer membranes, signals received and substrates bound are available to be transported across the cytoplasmic membrane using transport and signalling proteins imbedded there.

What information can be obtained from a simple stain?

A simple stain can provide information about the size, shape, arrangement, and basic characteristics of bacterial cells present in a sample. It can help differentiate between different types of bacteria based on their staining properties (e.g., gram-positive vs. gram-negative).

What is the measurement for cooking?

tablespoon vs gram

Can simple staining techniques be used to identify more than the morphological characteristics of microorganisms?

Yes, you can determine the arrangement of the micoorganism such as chain, random, or spiral as the simple staining allows you to obtained a distinctive contrast between background and its organisms being observed.

What are advantages and disadvantages of staining a sample?

Some cells produce gram-variable results. Some cells produce no results. The age of the culture can influence the results. It has limited usage in environmental biology and is not as good as molecular techniques.

Which stain gives the best clarity for each organism?

Different types of organisms require different types of staining techniques. The Gram stain is majorly used in the identification of a bacterial organism. This is considered an effective stain for microscopic organisms.

Short note on positive vs negative liberty?

Positive liberty is positive while negative liberty stands for negative

Grain of gold vs gram?

A grain of gold is a unit of weight traditionally used for precious metals like gold, equal to about 0.065 grams. A gram is a metric unit of weight equal to one thousandth of a kilogram. In comparison, a grain is much smaller than a gram.

What is more expensive platinum or diamonds?

Gram for gram, a gem-quality diamond is always more expensive than platinum. However, gram for gram, platinum is always more expensive that industrial diamonds. It is important to keep in mind that diamond prices are kept artificially high because DeBeers hides away much of the diamonds they mine to exaggerate scarcity i.e. diamonds are a manipulated market (vs. a free market).