Fecal coliforms have the ability to ferment lactose at 44C +/- 0.05C while regular coliforms ferment lactose at 35C-37C
The membrane filter / agar plates produce colonies from bacteria that can grow on the agar you are using (not all fecal coliforms can). The Colilert will turn color if the bacteria have the enzyme to metabolize substrates in the Colilert (not all do). If all fecal coliform bacteria could grow on the agar and all fecal Coliforms had the right enzymes you'd get the same numbers.
Coliform bacteria is associated with fecal matter, and since rain does not come in contact with fecal matter before hitting the ground, it should not contain coliform bacteria.
Coliforms are used as an indicator of water portability because they may be associated with the sources of pathogens that contaminates the water and they can be easily cultured and detected.
Total coliform (TC) guideline value is 50 TC/100mlFecal coliform (FC) guideline value is 200 E coli/100 mL or 200 fecal coliforms/100 mL
Yes,they generally come from from sewerage and are called 'fecal coliforms' since they are found in human and animal waste. Drinking contaminated water causes the bacterial diseases of Typhoid and Cholera.
Gram negative rods 2) Facultative anaerobes that ferment glucose. 3) Motility = peritrichous flagella 4) Enterics reside in the intestinal tracts of humans & animals as part of the normal flora. 5) Some thrive in rich soil. 6) Entero = intestine (small intestine). 7) Colo = colon, large intestine. 8) Coliforms = Enterics that ferment lactose (p.787). 9) Coliforms used as indicator of fecal pollution & potential fecal-borne pathogens.
Originally, fecal contamination was determined with the presence of coliform bacteria, a convenient marker for a class of harmful fecal pathogens. The presence of fecal coliforms (like E. Coli) serves as an indication of contamination by sewage. Additional contaminants include protozoan oocysts such as Cryptosporidium sp., Giardia lamblia, Legionella, and viruses (enteric). Microbial pathogenic parameters are typically of greatest concern because of their immediate health risk.http://en.wikipedia.org/wiki/Drinking_water
Coliforms are used as a indicator organism because they are abundant in matrix, easy to find, cost effective. Coliforms has frequently been used in water testing because of all of these benefits.
Eosin methylene Blue Agar contains peptone (a sugar of glucose), lactose, sucrose, and the dyes eosin Y and methylene blue. The sugars provide fermentable substrates to encourage growth of fecal coliforms. The dyes inhibit growth of Gram-positive organisms.
coliforms
looking for coliforms!