The four possible results after Gram staining are Gram positive, Gram negative, Gram variable and Gram in-determinant.
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
Gram stain
Hans Christian Gram invented this stain in 1884
Gram negative
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
It is possible to decolourise gram positive bacteria and thus get false results of all gram negative organisms. Similarly, it is possible to under-decolourise and get all gram positive organisms. Ideally controls of known organisms should be run with each stain.
A gram stain is a cow
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
Gram stain
Gram Negative
Lactococcus Lactus is a gram positive bacteria and therefore retains the darker staining and therefore shows on a gram stain as dark blue/violet colour. This is because the thick peptidoglycan cell wall retains the primary crystal violet stain.
Protists are often stained using a silver stain, not a Gram stain.
The Gram stain is used for bacteria and not for viruses.
Bacteria stain either gram-positive or gram-negative based on the presence or absence of a cell wall. Viruses do not pick up a gram stain.
Hans Christian Gram invented this stain in 1884
its the difference in cell wall of both the type of bacteria