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Why do all cells stain purple in the flagella stain but not in the gram stain?
Wiki User
∙ 9y ago
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
Wiki User
∙ 9y ago
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Does the gram stain procedure work for all bacteria?
Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.
You preformed a gram stain on a sample from a pure culture of bacteria and observed a field of red and purple cocci adjacent cells were not always the same color what do you conclude?
This is a Gram Stain. If the technique was proper, the red rods are Gram-negative and the purple cocci are Gram-positive. This staining technique is used to help identify various bacteria. The Gram-positive bacteria that are purple hold the stain due to it's layered cell membrane. It contains a peptidoglycan layer that acts as a lattice trapping the crystal violet-Iodine dye complex.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
What is the appearance of gram negative bacteria after addition of mordant in the gram stain?
Red/pink
What is the result of gram stain on human cell?
Human cells have no cell walls, only a plasma membrane, and as a result lack the peptidoglycan layer that gram stains utilize to differentiate between gram negative and gram positive species of bacteria. Therefore, human cells are unable to retain the crystal violet introduced in the first step of the gram stain, and stain negative. (they appear pinkish like gram-negative bacteria)
What color are gram negative cells?
Gram positive bacterial stain purple. They do not take up the counter stain.
Does the gram stain procedure work for all bacteria?
Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.
Why is decolorization necessary when performing a gram stain?
During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
Why does the counterstain not change the color of all of the cells is it because the primary stain repels or masks the secondary stain?
In a gram stain the primary stain is crystal violet. Iodine then sets that dye into the gram positive cells while alcohol washes out the crystal violet from the gram negative cells. Then safranin, which is the counterstain in a gram stain, is used to dye the rest of the bacteria. This is the example I can give you of why a counterstain does not change the look in all the cells. Though safranin stains all the cells, the gram positive cells that were dyed purple from crystal violet don't look pink - only the gram negative do.
Can you see spores in a gram stain preparation?
yes, spore will not stain , so it will shown as a empty space ( bright refrigent )inside the cells . the rest of the cells will be purple colored.
Do gram-positive cells carry a net positive charge?
Gram-positive does not refer to a positive charge, but to the purple color of the stain. Gram-negative bacteria do not retain the purple dye and are instead red in color.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What is the current theory about the mechanism of the gram stain?
The gram stain is a basic differential stain used to determine if a bacterial cell is gram positive or negative. Gram positive cells have a thick peptidoglycan layer that will trap the crystal violet iodine crystalls and apear purple. Gram negative cells only have a thin peptidoglycan layer that allows the crystals to diffuse out of the cell and will only be seen with the application of a counterstain, such as safranin which turns the cells pink.
What shape is pneumonia?
It is purple. The bacteria that cause it are gram-positive, which means that under the gram stain they become purple. Your lungs produce the lung stain when they are sick, so pneumonia is purple.
Which color is common to both the gram stain and the acid-fast stain?
purple
You preformed a gram stain on a sample from a pure culture of bacteria and observed a field of red and purple cocci adjacent cells were not always the same color what do you conclude?
This is a Gram Stain. If the technique was proper, the red rods are Gram-negative and the purple cocci are Gram-positive. This staining technique is used to help identify various bacteria. The Gram-positive bacteria that are purple hold the stain due to it's layered cell membrane. It contains a peptidoglycan layer that acts as a lattice trapping the crystal violet-Iodine dye complex.
What is the gram stain of gas gangrene?
yes yes gram stain is a purple color that makes takes gram crackers and makes them good to eat.
