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yes, spore will not stain , so it will shown as a empty space ( bright refrigent )inside the cells . the rest of the cells will be purple colored.
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Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
Is food poisoning negative or positive in a Gram stain?
There are a variety of bacteria that can cause foodborne illness: some are Gram-positive, others are Gram-negative. See Related Links.
Is it feasible to determine motility using a side prepared by the Gram Stain techniques?
Bacteria are hard to see if they are not stained, but if you stain them they are dead, so motility can not be determined.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Is varicella zoster virus gram negative of gram positive?
Gram-positive and gram-negative refer to the Gram stain used to prepare slides of bacteria for viewing under a light microscope. Viruses are too small to see under a light microscope and have to be prepared differently for viewing under an electron microscope. So the answer to your question is neither
What will you see of a gram stain of e coli?
E. coli is a gram negative bacteria, meaning that it has a cytoplasmic lipid membrane, a peptidoglycan layer, and a (LPS) lipopolysaccharide layer. As a result, e. coli stains a pink colour on a gram stain from the counterstain saffranin. Gram positives stain purple retain the crystal violet dye even after washed with a decolouring solution.
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
Of what use is the endospore stain?
The use of endospore stain is to see specialized cell structures. It can tell if some bacterium cells contain higher resistant spores within vegetative cells.
Is food poisoning negative or positive in a Gram stain?
There are a variety of bacteria that can cause foodborne illness: some are Gram-positive, others are Gram-negative. See Related Links.
What is the gram stain for the west nile virus?
There is no Gram stain for the rabies virus - it does not pick up either the stain or the counter-stain and has no official Gram stain status like bacteria do. When scientists are looking at slides of brains to see if an animal was infected with rabies, they use a special immunofluorescent stain made of antibodies against the rabies virus linked to either a vividly colored pigment or a fluorescent pigment. If the rabies virus is present, the antibodies in the stain adhere to the viral particles and then the pigment becomes fixed to the tissue as well, allowing the pathologist to "see" the virus (actually just that the virus is present and approximately where it is at - the virus is too small to see with a standard light microscope).
Is it feasible to determine motility using a side prepared by the Gram Stain techniques?
Bacteria are hard to see if they are not stained, but if you stain them they are dead, so motility can not be determined.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Is varicella zoster virus gram negative of gram positive?
Gram-positive and gram-negative refer to the Gram stain used to prepare slides of bacteria for viewing under a light microscope. Viruses are too small to see under a light microscope and have to be prepared differently for viewing under an electron microscope. So the answer to your question is neither
What is gram staining?
Gram Staining is a way to separate one large groups of bacteria into two. Crystal violet is used to dye the cells, those that retain the color are grouped as Gram-positive, and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic, making this process useful for detecting infections. A link is provided to permit a quick trip to the Wikipedia article on this topic.
Can Gram-positive organism appear as Gram-negative?
Staphylococcus are Gram positive because, in Gram's test, the decolorizing agent (ETOH) cannot penetrate the thick cell walls, and the stain remains behind: hence Gram positive. I am not aware of a gram negative staph species and, considering the degree of mutation that would be needed to form such a strain, I do not believe that it's possible. I should add that a not-so-brief scan of the net showed me no Gram negative staph mentioned anywhere. So -- for now -- no. That said, let's see what the future holds.
What is the gram stain for leptospira icterorrhsgiae?
Leptospira as a genus has characteristics of both Gram negative and Gram positive bacteria, in that they have a double membrane with peptidoglycan attached to the inner membrane. Thus when stained they could appear either positive or negative, if one could even see them at all. This in addition to their extreme slenderness makes visualization by gram stain less than ideal. Typically one would observe this organism by darkfield microscopy rather than attempt a likely to fail Gram stain.
