The use of endospore stain is to see specialized cell structures. It can tell if some bacterium cells contain higher resistant spores within vegetative cells.
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It is heated.
why is it necessary to perform an endospore stain to identify clostridium in health care settings
Malachite green
Vegetative cells readily decolorize because they are permeable.
One thing that endospore stains have in common with the acid fast stain is that heat primary stain penetration. Another thing that endospore stains have in common with acid fast stains are counterstain.
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
Negative cocci red
Gram staining protozoans yield variable results. Endospore, capsule, and AF stains will yield different results, as these stains are use on bacteria. Malaria is caused by a protozoan.
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
This paper will aid in keeping the stain over the bacterial smear.
because they cannot be differentiated from inclusions of stored material without a special stain .