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What prevents the cell from appearing green in the finished endospore stain?
Wiki User
∙ 14y ago
Vegetative cells readily decolorize because they are permeable.
Wiki User
∙ 14y ago
Wiki User
∙ 15y agoWhat prevents the vegetative cell from appearing green in the finished endospore stain?
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Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Why is it essential to apply heat during endospore staining?
because they cannot be differentiated from inclusions of stored material without a special stain .
What dye is used to stain endospores?
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
What so endospore stain have in common with the acid-fast stain?
One thing that endospore stains have in common with the acid fast stain is that heat primary stain penetration. Another thing that endospore stains have in common with acid fast stains are counterstain.
How would an endospore stain of mycobacterium appear?
Acid fast Mycobacterium have a waxy molecule in their cell wall that will take up and retain the malachite green stain when subjected to the endospore staining process. The uniformly green appearance of endospore stained acid fast cells doesn't mean they produce endospores. These are vegetative cells that have taken up color from the heat driving malachite green into their waxy cell wall.
How does the malachite green stain enter an endospore?
It is heated.
What dye is used to penetrate and stain an endospore?
Malachite green
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Why is it necessary to heat the malachite green?
Malachite green is used as a primary stain in bacteria. It is necessary to heat the malachite green in order to penetrate the endospore of the bacteria.
Why is it essential to apply heat during endospore staining?
because they cannot be differentiated from inclusions of stored material without a special stain .
What dye is used to stain endospores?
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
What so endospore stain have in common with the acid-fast stain?
One thing that endospore stains have in common with the acid fast stain is that heat primary stain penetration. Another thing that endospore stains have in common with acid fast stains are counterstain.
How would an endospore stain of mycobacterium appear?
Acid fast Mycobacterium have a waxy molecule in their cell wall that will take up and retain the malachite green stain when subjected to the endospore staining process. The uniformly green appearance of endospore stained acid fast cells doesn't mean they produce endospores. These are vegetative cells that have taken up color from the heat driving malachite green into their waxy cell wall.
What color are bacterial endospores after a gram stain is performed?
Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.
Prevents clotting of blood?
fresh green veggies and liver
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
Why it is difficult to burn green grass than dry grass?
In green grass, water is present, and this prevents it from burning.
