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Acid fast Mycobacterium have a waxy molecule in their cell wall that will take up and retain the malachite green stain when subjected to the endospore staining process. The uniformly green appearance of endospore stained acid fast cells doesn't mean they produce endospores. These are vegetative cells that have taken up color from the heat driving malachite green into their waxy cell wall.

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14y ago
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13y ago

Unstained endospores appear like a white, hollow, hole in the middle of the baccilli or whatever form of bacteria that you are observing. Note: only certain bacteria contain spores or are capable of forming spores..ex) clostridium or baccilli.....

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Q: How would an endospore stain of mycobacterium appear?
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What color are bacterial endospores after a gram stain is performed?

Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.


What is the purpose of staining an unknown organism?

By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.


Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?

Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin


What happens if you forget to add acetone to a gram negative stain?

When performing the Gram stain, acetone is used as a wash step between the iodine and safranin. By not washing, all stains will remain crystal violet purple, and none will appear safranin red/pink.


Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?

There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.

Related questions

How would an endospore stain of e coli appear?

Negative cocci red


Which stain would be the best choice for detecting mycobacterium the bacteria responsible for tuberculosis and leprosy?

ZN stain


What color are endospores after endospore stain?

After gram staining an endospore the color it would show up would be colorless or clear. It will not work for endospores because of its tough outer layer, stains can't penetrate.


What color are bacterial endospores after a gram stain is performed?

Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.


What is the purpose of staining an unknown organism?

By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.


Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?

Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin


What would cause a fluro pink stain to appear on wooden floor?

kids!


If Gram positive cells are not decolonize what color would they appear after completing the Gram stain?

pink


What types of conditions would an endospore be present?

When esstential nutrients are depleted!


What would happen if you placed a bacillus in a hot and dry environment?

Bacillus would form an endospore .


What happens if you forget to add acetone to a gram negative stain?

When performing the Gram stain, acetone is used as a wash step between the iodine and safranin. By not washing, all stains will remain crystal violet purple, and none will appear safranin red/pink.


If applying a green stain over a red stain what colour would I get?

You would get the color grey