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Acid fast Mycobacterium have a waxy molecule in their cell wall that will take up and retain the malachite green stain when subjected to the endospore staining process. The uniformly green appearance of endospore stained acid fast cells doesn't mean they produce endospores. These are vegetative cells that have taken up color from the heat driving malachite green into their waxy cell wall.
What else can I help you with?
What color are bacterial endospores after a gram stain is performed?
Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
What specimen from a TB patient would the acid fast stain be performed on?
The acid-fast stain is typically performed on sputum samples from TB patients to detect the presence of Mycobacterium tuberculosis. Other specimens that can be tested include bronchial washings, bronchoalveolar lavage fluid, and tissue biopsies.
What is the purpose of staining an unknown organism?
By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the PH would be what?
lol, we must be in the same class, b/c i have all of the same questions. did u figure out the answer? I think its a base ... qc the microbiology lab Cute, from the best that I could find some sources say pH 7 and others had given a pH 8. But could not find a solid answer. As a simple stain it would have to have a pH of <7. As we know, the charge on the cell is always going to be negative (an anion). In order to achieve a simple stain we want the stain attracted to the cell wall; therefore, the stain must be positively charged (a cation) for the opposite forces to attract. The more acidic the compound - the smaller its pH number with 7 being neutral. As we know, the [H]+ increases as the pH decreases becoming evermore positively charged. Simple answer: <7
How would an endospore stain of e coli appear?
Negative cocci red
Which stain would be the best choice for detecting mycobacterium the bacteria responsible for tuberculosis and leprosy?
ZN stain
What color are bacterial endospores after a gram stain is performed?
Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.
What color are endospores after endospore stain?
After gram staining an endospore the color it would show up would be colorless or clear. It will not work for endospores because of its tough outer layer, stains can't penetrate.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
If Gram positive cells are not decolonize what color would they appear after completing the Gram stain?
pink
What types of conditions would an endospore be present?
When esstential nutrients are depleted!
What specimen from a TB patient would the acid fast stain be performed on?
The acid-fast stain is typically performed on sputum samples from TB patients to detect the presence of Mycobacterium tuberculosis. Other specimens that can be tested include bronchial washings, bronchoalveolar lavage fluid, and tissue biopsies.
What would happen if you placed a bacillus in a hot and dry environment?
Bacillus would form an endospore .
What is the purpose of staining an unknown organism?
By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.
What is an antonym for endospore?
Well, since an endospore is "inside", I would vote for an exospore, meaning "outside".Endospore means a small asexual spore that develops inside the cell of some bacteria and algae. Since this formation is inside the cell then, so I would say that Outside, outer, exit should be the words related to it.I am also preparing for GRE and I have been taking verbal ability practice tests and live review classes on examville, my scores are already improving.
If applying a green stain over a red stain what colour would I get?
You would get the color grey
