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Leptospira as a genus has characteristics of both Gram negative and Gram positive bacteria, in that they have a double membrane with peptidoglycan attached to the inner membrane. Thus when stained they could appear either positive or negative, if one could even see them at all. This in addition to their extreme slenderness makes visualization by gram stain less than ideal. Typically one would observe this organism by darkfield microscopy rather than attempt a likely to fail Gram stain.

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Q: What is the gram stain for leptospira icterorrhsgiae?
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