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What is gram staining?

Updated: 4/27/2022
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16y ago

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Gram Staining is a way to separate one large groups of bacteria into two. Crystal violet is used to dye the cells, those that retain the color are grouped as Gram-positive, and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic, making this process useful for detecting infections. A link is provided to permit a quick trip to the Wikipedia article on this topic.

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16y ago
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15y ago

Gram staining is a technique used to helpdifferentiate bacteria by their ability to hold onto a stain. Also the staining process makes it possible to see bacteria clearly under a microscope. Simply put, Gram staining works like this: An initial stain has been applied to a fixed bacterial smear on a slide. The initial stain is rinsed and a second stain is applied. The second stain has been rinsed and the slide is observed under the microscope. If the bacterial smear retains the color of the initial stain (usually crystal violet stain), it is considered gram positive. If the smear looses its initial stain and retains the color of the second stain (ex safrinin or carbol fuchin) it is considered gram negative. This technique is named after the man who discovered this method in 1884. His name was Christian Gram.

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13y ago

The Gram's stain is done by smearing part of a colony onto a microscope slide.

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Related questions

Is gram staining related to flagella?

No. It is a staining on the cell itself.


Who developed Gram staining?

Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)


Why gram staining classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


Why is Gram staining classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


Why is gram staining important?

It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.


What is staining for candida albicans?

gram positive


Why is safrinin used in gram staining?

as a couterstain


What are the colony characteristics and Gram staining of bacillus circulans?

Gram Positive Rods


Is gram staining special staining?

yes it is a special staining like other types....its importance is that one can identify the type of bacteria........


What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?

A basic dye used in gram staining is crystal violet.


Does giardia show up on gram stain?

no, it's eukaryotic. Gram staining is for bacteria.


What is the staining process for separating bacteria?

This is known as a gram test.