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What are enzymes cutting DNA at specific sites to form restriction fragments called?
Wiki User
∙ 7y ago
restriction enzymes or endonuclease enzymes
Wiki User
∙ 11y ago
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Why ligation can be considered the reverse of the restriction enzyme process?
Restriction enzymes cuts out a specific short nucleotide sequence while as the process of ligation, DNA ligase joins them together. So ligase can be considered the reverse of the restriction enzyme process as it joins DNA fragments together instead of cutting them out.
Which best describes the role of restriction enzymes in the analysis of eDNA?
cutting large DNA molecules into smaller pieces.
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
Why do the number of DNA fragments and the length of each fragment produced by RFLP analysis differ from person to person?
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
What do biotechnology use to cut DNA molecules at specific sequences?
The cutting of DNA at specific location became possible with the discovery of the so-called 'molecular scissors' i.e. restriction enzymes.
What does a geneticist use to cut DNA at specific base sequences?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into smaller fragments. Restriction enzymes are found in bacteria, where they act like molecular scissors by cutting up DNA from invading viruses or bacteriophages. Each restriction enzyme recognizes a specific nucleotide sequence and cuts the DNA at that site. This process makes restriction enzymes extremely useful in biotechnology where they are used in procedures such as DNA cloning, DNA fingerprinting, and genetic engineering. There are hundreds of known restriction enzymes, and each one was named for the bacteria from which it was isolated. For example, EcoRI was isolated from Escherichia coli and HaeIII from Haemophilus aegyptius.
What cuts DNA into fragments?
cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
Why was the discovery of restriction enzymes important to recombinant DNA technology?
Recombinant DNA technology requires fragments of DNA from the source genome. Using crude methods such as mechanical shearing, we get random fragments of DNA, and their sequence is unknown. Restriction enzymes are specific in site recognition and cutting and their discovery lead to proper fragments of DNA which have some known sequences.
What process is used to cut DNA into fragments?
DNA can be fragmented using restriction endonucleases or restriction enzymes. Restriction enzymes identify specific sequences within the DNA and cause cleavage generating fragments. When this digested DNA is allowed to run in gel electrophoresis fragments get separated according to their mass. When visualized under UV transilluminator, fragmented DNA can be observed as fluorescing bands.
Why ligation can be considered the reverse of the restriction enzyme process?
Restriction enzymes cuts out a specific short nucleotide sequence while as the process of ligation, DNA ligase joins them together. So ligase can be considered the reverse of the restriction enzyme process as it joins DNA fragments together instead of cutting them out.
What does restriction endonuclease do?
They cut DNA at specific sequences. Restriction endonucleases work by cutting DNA at specific sequences. The places that are cut are known as restriction sites.
What are used to cleave DNA into fragments?
Restriction enzymes. Babe
Which best describes the role of restriction enzymes in the analysis of eDNA?
cutting large DNA molecules into smaller pieces.
Do restriction enzymes cut protein molecules at specific sites?
No. restriction enzymes do not cut proteins. restriction enzymes cut DNA molecules at specific sites called restriction sites.
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
Why do the number of DNA fragments and the length of each fragment produced by RFLP analysis differ from person to person?
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
Which restriction enzymes cuts the smallest pieces of DNA?
In a practical application, we need a Book; it will also say which restriction enzymes leave the longest fragments.
