0
Why was the discovery of restriction enzymes important to recombinant DNA technology?
Wiki User
∙ 12y ago
Recombinant DNA technology requires fragments of DNA from the source genome. Using crude methods such as mechanical shearing, we get random fragments of DNA, and their sequence is unknown. Restriction enzymes are specific in site recognition and cutting and their discovery lead to proper fragments of DNA which have some known sequences.
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
What information is not given by a restriction map?
Restriction mapping is the most detailed thing that can be done with a segment of the DNA.It gives valuable detail about the gene regulating sequence and the introns.Restriction enzymes ans DNA ligase are important in making recombinant DNA.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
Why is the jj Thomson discovery important?
it wasnt..................
What are the recombinant DNA is formed by joining DNA molecules?
In rDNA technology, a plasmid molecule serves as a vector in which the insert or gene of insert is ligated to for chimeric DNA or recombinant DNA. It is an important step in DNA cloning. Thus formed chimeric vector can be propagated in E.coli for large production of clones. If you clone the gene in an expression vector you can express proteins in the host bacterium.
Why was niels bohr discovery important?
to help us have sex
What is the definition of technology important of science and technology?
Science discovers and Technology makes things from the discovery
Which technology was important to the discovery of the cell theory?
The Microscope.
What information is not given by a restriction map?
Restriction mapping is the most detailed thing that can be done with a segment of the DNA.It gives valuable detail about the gene regulating sequence and the introns.Restriction enzymes ans DNA ligase are important in making recombinant DNA.
How does science and technology help us to travel?
Discovery of wheel is most important discovery for travelling . Then invension of motor. These are two most important invensions which helps us to travel long distance easily. This can be possible only because of science and technology.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
When a restriction enzyme cuts a dna molecule the cuts are staggered so that the dna fragments have single stranded ends why is this is important in recombinant dna work?
It is important because the fragments will bond to other fragments with complementary single-stranded ends.
What important discovery was made there?
What important discovery was made there
How are restriction enzymes important tools in genetic engineering?
restriction enzymes are important tools in genetic engineering because they just are!!
Was glass an important discovery?
Yes glass was an important discovery
What is sentence for significanse?
The significance of the discovery led to a breakthrough in medical research.
Why is the northwest passage still an important discovery?
why is the northwest passage have been an important discovery
Where in the world did Darwin His first important discovery.?
Charles Darwin made his first important discovery in Argentina.
