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What are the three enzymes involved in DNA transcription?
The three enzymes involved in DNA transcription are RNA polymerase, helicase, and topoisomerase. RNA polymerase is responsible for synthesizing RNA from a DNA template, helicase unwinds the DNA double helix, and topoisomerase helps to relieve the tension that builds up ahead of the replication fork.
Difference between DNA replication and transcription?
DNA replication is the process by which DNA makes an identical copy of itself, creating two identical DNA molecules. Transcription is the process by which a segment of DNA is copied into RNA by RNA polymerase, resulting in the creation of mRNA. In summary, DNA replication produces a copy of the entire DNA molecule, while transcription produces a copy of specific genes or segments of DNA in the form of mRNA.
What are the 4 enzymes in DNA replication?
DNA Helicase - responsible for separating the two stands DNA Polymerase - responsible for catalyzing the addition of bases to the new strand DNA Ligase - responsible for sealing fragments
The life cycle of a typical DNA virus consists of 4 steps?
The life cycle of a typical DNA virus consists of 7 steps. The steps are entry, uncoating, early transcription, viral DNA replication, late transcription, viral assembly, and the virus exits.
The DNA of a human cell can be cut and rearranged by using?
The answer as of Castle Learning was choice 4, Enzymes.
DNA strands can be clipped crosswise at selected positions by using enzymes called?
DNA can be cut into smaller fragments by enzymes (which are proteins) known as restriction endonucleases (REN's). These enzymes are sequence specific - meaning they produce a cut only at a particular site on the DNA strand. This site where the cut is produced is called the restriction site. Restriction sites are 4 - 6 nucleotides in length. Every restriction enzyme has a different restriction site. This property allows researchers to treat two different DNA samples with the same set of restriction enzymes and then analyze the resulting fragments.A. DNA finger printing
The following steps must be performed to make a bacterium produce human protein X?
1 is translation. 2 is restriction enzyme. 3 is prokaryotic transcription. 4 is DNA ligase. 5 is transformation. 6 is eukaryotic transcription. 7 is reverse transcription. So the order of the steps based off of the numbers are 6,7,2,4,5,3,1.
At the DNA synthesis G2 checkpoint DNA replication is checked by 1 receptor proteins 2 Repair enzymes 3 electron transport chains 4 cell surface markers?
2 Repair enzymes. At the DNA synthesis G2 checkpoint, DNA replication is checked by repair enzymes that detect and repair any mistakes in the replicated DNA before the cell progresses to mitosis. Receptor proteins, electron transport chains, and cell surface markers are not directly involved in checking DNA replication at this checkpoint.
Describe the 5 steps in protein synthesis?
1. The DNA unzips 2. The mRNA reads the code (codon) of the DNA (Transcription) 3. The mRNA moves out of the nucleus and enters the cytoplasm to attach to the ribosomes 4.tRNA brings amino acids to the ribosomes according to the original DNA 5. The amino acids bind together and are released into the cytoplasm as a protein
Why you freeze the pellet during DNA isolation?
Freezing the pellet helps to break open the cells and release the DNA molecules. It also helps to create a more uniform suspension that can result in better DNA yield and quality during the isolation process.
Name the 4 bases involved in DNA replication?
The four bases involved in DNA replication are adenine (A), thymine (T), cytosine (C), and guanine (G). These bases pair up specifically (A with T and C with G) during DNA replication to ensure accurate copying of the genetic information.
Where are enzymes active in the body at pH 4 and 5?
Liver enzymes- Transaminase
