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Karl Fisher Reagent

Dissolve 762 g of iodine in 2,420 ml of pyridine in a 10 liter glass stoppered bottle, and add 6 liters of methanol. To prepare the active stock, add 3 liter of the foregoing stock to a 4 liter bottle, cool in ice bath. Add carefully 135 ml of liquid sulfur dioxide, collected in a calibrated cold trap,and stopper the bottle. Shake the mixture until homogeneous, and set aside for one or two days before use.

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Q: What are the constituents of Karl Fisher reagent?
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What is the use of Karl fisher reagent?

Karl fisher reagent ia mixture of dehydrated methanol,iodine sulphur dioxide and pyridine. but now a days, due to extremely toxic behaviour of pyridine, it is usuallly replaced by amines


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