Karl fisher reagent ia mixture of dehydrated methanol,iodine sulphur dioxide and pyridine. but now a days, due to extremely toxic behaviour of pyridine, it is usuallly replaced by amines
Karl Fisher Reagent Dissolve 762 g of iodine in 2,420 ml of pyridine in a 10 liter glass stoppered bottle, and add 6 liters of methanol. To prepare the active stock, add 3 liter of the foregoing stock to a 4 liter bottle, cool in ice bath. Add carefully 135 ml of liquid sulfur dioxide, collected in a calibrated cold trap,and stopper the bottle. Shake the mixture until homogeneous, and set aside for one or two days before use.
because pyridine is toxic...so amines are used in place of pyridine.......
To calculate the Karl Fischer factor there are three factors that are required. The ml KF reagent used for sample, the factor mg H20/ml KF reagent and the weight in grams.
no
No
Karl Fisher Reagent Dissolve 762 g of iodine in 2,420 ml of pyridine in a 10 liter glass stoppered bottle, and add 6 liters of methanol. To prepare the active stock, add 3 liter of the foregoing stock to a 4 liter bottle, cool in ice bath. Add carefully 135 ml of liquid sulfur dioxide, collected in a calibrated cold trap,and stopper the bottle. Shake the mixture until homogeneous, and set aside for one or two days before use.
because pyridine is toxic...so amines are used in place of pyridine.......
To calculate the Karl Fischer factor there are three factors that are required. The ml KF reagent used for sample, the factor mg H20/ml KF reagent and the weight in grams.
Jens Malte Fisher has written: 'Karl Kraus'
Karl Fisher Titration measures only the water content in a product sample. Loss on drying measures the total loss in weight of a material as a result of drying.?æ
1)they are use to form laboratory salt when combined with acid. 2)they are used to detect the chemical reagent. 3)they are use to manufacture many laboratory reagent.
Use a pipette.
yes
no
No
antioxident BY USE U.V
You are not supposed to use buret reagent to detect sugar carbohydrates in a solution. Buret reagent is used to detect proteins. Try using iodine ( I2KI)