When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
Freezing doesn't effect the enzymes since freezing does not permanently affect enzyme structure. Boiling permanently changes the structure and can change the enzymes.
An allosteric inhibitor stops enzyme activity by binding to an allosteric site and causing the conformation of the enzyme to change.
The concentration of hydrogen ions in solution affects the enzyme activity. Each enzyme has maximal efficiency under an optimum pH. Since pH is one of the factors for the denaturation of proteins, if an enzyme is submitted to a pH level under which it is denatured there will be no enzymatic activity.
Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive
When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.
Increase the amount of substrate for the enzyme.
"During this experiment, we will be looking to see the effects of heat on enxyme activity" "This stain remover contains an enzyme"
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
Denature enzyme activity
activators; inhibitors
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
Enzyme activity is affected by other molecules, temperature, chemical environment (e.g., pH), and the concentration of substrate and enzyme. Activators are molecules that encourage enzyme activity, and inhibitors are enzymes that decrease enzyme activity. Sometimes a cofactor is necessary for the enzyme to work.
Freezing doesn't effect the enzymes since freezing does not permanently affect enzyme structure. Boiling permanently changes the structure and can change the enzymes.
inhibitor
Enzyme activity increases with temperature, but only up to a point.