TSI - Alkaline slant/Acid butt with H2S
SIM - Motile, H2S positive
Urease - Negative
imvic test helps in identi fication of salmonello typhi and a specific agar named salmonella shigella agar is used for culturing these species
IMVIC farmula for e.coli is ++--
it should test + for catalase but Salmonella isolates are moderate catalase reactors.
If a blood test comes back with salmonella typhi O at a level of 1 80, it means a person has contracted typhoid fever.
mywadil test is 1/160 of Para - typhi AH
Staphylococcus epidermidis indole test - negative methyl red - negative voges proskauer test - positive citrate test - no idea
salmonella typhi H is a typhoid count. it is very dangerous. in typhi H it tells us that the count of the disease will decrease very slowly and it will take years time to decrease but in typhi O it tells us that the count will decrease in a short period of time such as in 1 or 2 weeks. the count 1/80 tells we are at a minimum level. that means we have typhoid tYphi O,but if its above the count it is very bad. if you have temprature or shiviring and vomitting you should immediatly go to the doctor for a blood test to ensure safety. remember one thing if you have typhoid you should take a diet.do not eat anything solid only take liquids.such as soups. i have also got typhoid and this is what he doctor said to me and i had typhi O.and in typhoid you should take rest for at least a week.
Marker for Widal Test carried out for the detection of Salmonella
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
You can differentiate between different salmonella strains using serology. Each strain has its own combination of antigens. Depending on the O, H, and vi antigens, you can determine which strain is present. The O-antigen is the somatic polysaccharide antigen, the H-antigen is the flagellar antigen and the vi-antigen is the capsular antigen. Antibodies to most of the variants of these antigens are available commercially for use in labs. Adding an aliquot of specific antisera to a suspension of your salmonella spp, and incubating it for a few hours in a water bath will result in visible agglutination if your salmonella strain possesses the specific antigen to the antibody you added. For example, Salmonella typhi will show agglutination with O [9,12], H [d] and vi.
The Widal test is a presumptive (positive) serological test for enteric fever or undulant fever (typhoid fever) if the antigen titer is more than 1:160 and if the person tested had past infections or is immunized. More tests should be done since it is now 1:320. If the next titer comes back 4x higher, then the person has an active case of typhoid fever.