Unzipping, Complementary base pairing, Backbone formation.
Also known as Initiation, Replication and Termination.
1. Interphase: replication and growth of the chromosomes occur.
2. Prophase: the chromatin condenses to see the individual chromosomes and centrioles move toward the opposite poles of the cell and spindle fibers grow.
3. Metaphase: the chromosomes align in the center of the cell.
4. Anaphase: spindles attach to the chromosomes and split them in part towards the opposite poles.
5. Telophase: the cell begins to separate into to new daughter cells.
6. Cytokinesis: the nucleus begins to form and the cells are completely cut off from each other.
DNA replicationbegins with the unzipping of the parent molecule as the hydrogen bonds between the base pairs are broken. Once exposed, the sequence of bases on each of the separated strands serves as a template to guide the insertion of a complementary set of bases on the strand being synthesized. The new strands are assembled from deoxynucleoside triphosphates. Each incoming nucleotide is covalently linked to the "free" 3' carbon atom on the pentose as the second and third phosphates are removed together as a molecule of pyrophosphate (PPi). The nucleotides are assembled in the order that complements the order of bases on the strand serving as the template. Thus each C on the template guides the insertion of a G on the new strand, each G a C, and so on. When the process is complete, two DNA molecules have been formed identical to each other and to the parent molecule.
First, break the hydrogen bond between bases of the two antiparallel strands. Next, bind the RNA Primase. Next is the elongation process. Then, read the fragments and remove the RNA Primers. Finally, the last step is termination.
Primer made from primase attracts transcription factors, which call DNA-dependent DNA polymerase.
DNA-dependent DNA polymerase binds while helicase unwinds the helix.
Stable single-stranded DNA prevent the helix from re-annealing.
DNA polymerase adds nucleotides in a 5' to 3' direction.
RNAase replaces the RNA primer
Another DNA polymerase replaces nucleotides.
Ligase joins them together.
There is G1, G2, S(synthesis), and M(mitosis) phase.
(Correct Order)
G1 is the phase where the cell preps for separation
S is the synthesis phase, this phase is when the cell replicates
G2 is the phase when the cell duplicates all of the chromosomes
M or mitosis phase is the phase in which the one cell divides into 2 daughter cells
http://www.dnareplication.info/stepsofdnareplication.php
Nucleus takes place during DNA replication.
Helicase unwinds double helix
Polymerase adds nucleotides
Ligase binds Okazaki fragments
Helix bonds again
It has to be heated and u know whatever
The melting is important.
To produce metal from an ore, the ore must be mined, or removed from the ground. Then the ore must be processed to extract the metal.
1)The first major step for the DNA Replication to take place is the breaking of hydrogen bonds between bases of the two antiparallel strands. The unwounding of the two strands is the starting point. The splitting happens in places of the chains which are rich in A-T. That is because there are only two bonds between Adenine and Thymine (there are three hydrogen bonds between Cytosine and Guanine). Helicase is the enzyme that splits the two strands. The initiation point where the splitting starts is called "origin of replication".The structure that is created is known as "Replication Fork".2) One of the most important steps of DNA Replication is the binding of RNA Primase in the the initiation point of the 3'-5' parent chain. RNA Primase can attract RNA nucleotides which bind to the DNA nucleotides of the 3'-5' strand due to the hydrogen bonds between the bases. RNA nucleotides are the primers (starters) for the binding of DNA nucleotides.3) The elongation process is different for the 5'-3' and 3'-5' template.a)5'-3' Template: The 3'-5' proceeding daughter strand -that uses a 5'-3' template- is called leading strandbecause DNA Polymerase ä can "read" the template and continuously adds nucleotides (complementary to the nucleotides of the template, for example Adenine opposite to Thymine etc).b)3'-5'Template: The 3'-5' template cannot be "read" by DNA Polymerase ä. The replication of this template is complicated and the new strand is called lagging strand. In the lagging strand the RNA Primase adds more RNA Primers. DNA polymerase å reads the template and lengthens the bursts. The gap between two RNA primers is called "Okazaki Fragments".The RNA Primers are necessary for DNA Polymerase å to bind Nucleotides to the 3' end of them. The daughter strand is elongated with the binding of more DNA nucleotides.4) In the lagging strand the DNA Pol I-exonuclease- reads the fragments and removes the RNA Primers. The gaps are closed with the action of DNA Polymerase (adds complementary nucleotides to the gaps) and DNA Ligase (adds phosphate in the remaining gaps of the phosphate - sugar backbone).Each new double helix is consisted of one old and one new chain. This is what we call semiconservative replication.5) The last step of DNA Replication is the Termination. This process happens when the DNA Polymerase reaches to an end of the strands. We can easily understand that in the last section of the lagging strand, when the RNA primer is removed, it is not possible for the DNA Polymerase to seal the gap (because there is no primer). So, the end of the parental strand where the last primer binds isn't replicated. These ends of linear (chromosomal) DNA consists of noncoding DNA that contains repeat sequences and are called telomeres. As a result, a part of the telomere is removed in every cycle of DNA Replication.6) The DNA Replication is not completed before a mechanism of repair fixes possible errors caused during the replication. Enzymes like nucleases remove the wrong nucleotides and the DNA Polymerase fills the gaps.Similar processes also happen during the steps of DNA Replication of prokaryotes though there are some differences.
They take place in a cell
Replication does not take place during mitosis. It takes place before meiosis begins.
DNA replication occurs during the synthesis phase of interphase of the cell cycle.
replication in the cell take place in the second phase which is the metaphase.
No, DNA replication takes place inside the nucleus during S phase of cell cycle.
DNA replication takes place in the chromosome which is located in the nucleus of a cell.
During S (synthesis) phase, between the G1 and G2 phases.
"DNS" does not replicate.
the nucleus
DNA replication occurs during the S or synthesis stage of interphase.
genomic replication, or DNA replication, is performed by multiple enzymes that together are called a replicase. This replication takes place within the nucleus of a cell
cause it make the replication go faster
i think interphase