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It depends on who is performing the procedure. If a medical doctor is performing the procedure, then yes it will be as sterile as possible with clean instruments, surgical gloves, etc. If a religious authority is performing it (rabbi, etc.), it is probably not a sterile procedure.
a sample of pathogens
Sterile mineral oil is used to prevent the loss of moisture in the stock culture.
The conditions that allow you to culture tissue are also ideal for the growth of bacteria, which given a chance will devour your tissue culture.
When performing a culture for free fluid in the abdomen, you would typically request sterile containers for the fluid sample, appropriate culture medium for microbiological testing, culture swabs for collection, and possibly syringes or needles for aspiration if needed. It's also essential to have appropriate personal protective equipment (PPE) like gloves and a lab requisition form for labeling purposes.
Apply disinfectant and use sterile materials.
To prevent moisture loss. A.C.
Yes, you should always be sterile before performing any experiment as to not add any variables.
A pure culture contains only one bacteria and no other. A sterile culture has only one bacteria in it and is usually used for transport. Contamination is something that doesn't belong. It can even be a small bit of plastic or wire. It is the opposite of a pure culture. The whole idea when working with isolating bacteria is to figure out which bacteria (or microbe) is causing an infection or disease in a patient. Your job is to help to figure this out so that the proper treatment can be started as soon as possible.
HLA Phenotyping, Paternity & DNA testing.
A sterile container must be used to collect a urine culture for many reasons. The sample collected cannot be tainted with any substance that is being tested for.
Yellow top tube is used for collecting blood for blood culture (SPS) tests. It is sterile.