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The 4 steps of genetic engineering are DNA cleavage and isolation, production of recombinant DNA cloning, and screening. DNA cleavage and isolation uses restriction endonucleases to break up DNA strands. The production of recombinant DNA inserts DNA fragments into plasmids or similar vectors also digested by same restriction enzymes.

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9y ago
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9y ago

DNA extraction from cell

using restriction enzyme to cut the Large segment of DNA is one important step,

injection of foreign DNA segment to a cell involving some techniques such us electroporation, micro-injection or by virus vectors.

Integrase it used to fuse foreign DNA segment to the DNA of desired cell
7 steps

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10y ago

Sorry but these are not in order but they are right

The plasmid is cut with restriction enzymes.

As the bacterium divides, the doner gene is cloned.

A doner gene is spliced into the plasmid.

A plasmid is removed form the bacterium.

The bacterium transfers the doner gene to other organisms it infects.

The altered plasmid is returned to the bacterium.

-Halle-

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13y ago

Bacteria divide rapidly making

copies of the human insulin gene

Bacterial cells use the DNA code for insulin to make the human insulin protein

Use enzymes to cut the insulin gene out of human DNA

Transfer the insulin gene to bacteria

Insulin protein is purified and used to treat diabetic patients

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11y ago

1-Isolate the target gene of species 1

2-inject the isolated gene into the genome of species 2 's zygote.

3-integration of the gene occcurs

4-obtain a transgenic individual

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14y ago

Cleaving DNA, Recombining DNA, Cloning, Screening.

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14y ago

i dont know the best way to know is bring ur science book home and work hard or ask a teacher i suggest work hard and bring ur science book home.............. thank u

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12y ago

Clone a gene from one organism.

Put the gene into a different organism.

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Q: What are the steps involved in genetic engineering?
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