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What can happen if a sample was immersed in the chromatography solvent instead of suspended above it?
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Why stop paper chromatography from reaching the top of the paper?
The end of the paper nearest the spot is allowed to be in contact with a suitable solvent, WITHOUT immersing the spot itself. If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself.
What is the difference between vacuum chromatography and flash chromatography?
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
What does it mean if the spot is in the solvent front in chromatography?
it means that the solvent dissolves the solute.
What are the relations with pigment and chromatography?
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
What is the moving solvent called in chromatography?
Mobile phase.
Why stop paper chromatography from reaching the top of the paper?
The end of the paper nearest the spot is allowed to be in contact with a suitable solvent, WITHOUT immersing the spot itself. If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself.
Why can the pigment spot on chromatography paper not be submerged in solvent?
This is because the nonpolar pigments would just simply dissolve in the nonpolar solvent instead of traveling up the TLC plate.
What is the difference between vacuum chromatography and flash chromatography?
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
What does it mean if the spot is in the solvent front in chromatography?
it means that the solvent dissolves the solute.
What are the relations with pigment and chromatography?
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
Why solvent is used in Paper chromatography?
In chromatography you need one stationery phase (paper) and one mobile phase (here solvent, moving by capillary forces)
What is the moving solvent called in chromatography?
Mobile phase.
Is methanol a good solvent to use in paper chromatography?
Because methanol is a very polar solvent
What would the result be if while carrying out TLC chromatography you used a solvent of 200 is to 1 ethyl acetate and aqueous ammonia instead of 200 is to 1 ethyl acetate and acetic acid?
chromatography is when two substances separate. iprdufwjiwreusdoi
What uses electrostatic attraction to separate a solute from a solvent?
Chromatography
What is the best solvent for phenol for use in Gas Chromatography?
water
What is meant by tailing a spot in Chromatography?
Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.
