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Why can the pigment spot on chromatography paper not be submerged in solvent?
Wiki User
∙ 13y ago
This is because the nonpolar pigments would just simply dissolve in the nonpolar solvent instead of traveling up the TLC plate.
Wiki User
∙ 13y ago
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Why solvent is used in Paper chromatography?
In chromatography you need one stationery phase (paper) and one mobile phase (here solvent, moving by capillary forces)
In chromatography why do some colours not spread up the paper?
They do not move up because the aren't soluble the solvent
Why was a ball pen not used to draw the line?
In chemistry, a pen is not used to draw lines on chromatography paper. A pencil is used to draw lines on the paper because the ink can smear and interfere with the chromatography and the ink will dissolve in the chromatography solvent.
What do the Rf values indicate about the relative solubility of the pigment in the the solvent?
The Rf values do not indicate the solubility of a substance. The Rf value or retardation factor is the ratio of the distance traveled by the center of a pot to the distance traveled by the solvent front in chromatography.
What would happen if you submerge the chromatography paper above the pencil line?
Try and see: if it wouldn't be above the level of solvent, the spots will run off.
What are the relations with pigment and chromatography?
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
What determines the distance a pigment moves up the paper?
A non polar solvent will determine the distance in which a pigment moves up a paper. This will only work on chromatography paper.
Which determines the speed with which a pigment moves up the chromatography paper?
Chromatography is a technique that separates molecules from each other on the basis of their solubility in particular solvents. As a nonpolar solvent moves up the chromatography paper, the pigment moves along iwth it. The more non-polar a pigment, the more soluble it is in a nonpolar solvent, and the faster and father it proceeds up the chromatography. Pg 94, laboratory 8.1, Inquiry into Life, Sylvia S. Mader, laboratory manual, 12th edition
3 Why do the pigments become separated during the development of the chromatogram?
The chromatogram, or chromatography paper has solvent on it. Each pigment will separate itself from the others due to the relative solubility of that particular pigment.
What pigment is most soluble?
assuming you used an ether based chromatography solution it will be the pigment that is at the top of the chromatography paper
Why solvent is used in Paper chromatography?
In chromatography you need one stationery phase (paper) and one mobile phase (here solvent, moving by capillary forces)
What two factors determine the rate at which the pigments travel up the chromatography paper?
Rf value. polarity of solvent
How is chromatography used to study plant pigment?
chromatography is basically a technique used for the separation of different components.... plant pigment consist of different components..... the sample is taken nd spotted over a chromatography paper..... nd den it is kept in suitable solvent to get separated...... different components travel different distance on chromatogram.... dis is how v cn use chromatography to study plant pigments...
Is methanol a good solvent to use in paper chromatography?
Because methanol is a very polar solvent
What is the purpose of the solvent in chromatography?
The basic purpose of the solvent is to move the pigments up the chromatography paper through capillary action so that the pigments can be separated. The basic importance of chromatography is to separate different solvents by their molar mass. In doing so, one can separate the different pigments within a solution and measure how much of each pigment is present.
What will happen if there is an overrun of solvent in paper chromatography?
The Rf value cannot be determined since the distance of the solvent may overlay in the paper.
A method of separating mixtures by using chromatographic paper and a solvent?
This method is called paper chromatography.
