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Try and see: if it wouldn't be above the level of solvent, the spots will run off.

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Why must pencil line be drawn above the level of solvent?

Drawing a pencil line above the level of the solvent ensures that the compound being separated does not dissolve directly in the solvent, preventing interference with the separation process. This helps maintain a distinct separation between the solvent front and the compounds being separated, leading to more accurate results during chromatography.


Why must the level ofthe solvent not reach the pencil line where the spots are?

If the solvent level reaches the pencil line where the spots are, it can dissolve the compounds in the mixture, causing them to spread out and overlap. This can lead to inaccurate results and difficulties in analyzing the separated components on the chromatogram. It's important to maintain the separation of the compounds on the chromatography plate for clear identification.


What would happen if the solvent is above the level of initial sample spots?

If the solvent is above the level of the initial sample spots in a chromatography experiment, it can lead to the sample being dissolved or dispersed throughout the solvent, causing smudging of the spots and inaccurate separation of components. This can result in poor resolution and difficulty in analyzing the components present in the sample.


Why is the mixture placed above the level of the solvent in chromatography?

Placing the mixture above the solvent level in chromatography allows the solvent to travel up the stationary phase through capillary action, carrying the components of the mixture at different rates based on their interactions with the stationary phase. This separation process helps to distinguish and isolate the different components of the mixture.


Why must the solvent level be below the colored spots in chromatography?

If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.

Related Questions

Why must pencil line be drawn above the level of solvent?

Drawing a pencil line above the level of the solvent ensures that the compound being separated does not dissolve directly in the solvent, preventing interference with the separation process. This helps maintain a distinct separation between the solvent front and the compounds being separated, leading to more accurate results during chromatography.


How do you set up paper chromatography?

To set up paper chromatography, begin by cutting a strip of chromatography paper and marking a baseline about 1-2 cm from one end using a pencil. Next, apply small spots of the sample solution along the baseline, ensuring they are spaced apart. Then, place the paper in a developing chamber with a shallow layer of solvent, ensuring the baseline is above the solvent level. Finally, cover the chamber to prevent evaporation and allow the solvent to rise up the paper, separating the components of the sample.


Why must the level ofthe solvent not reach the pencil line where the spots are?

If the solvent level reaches the pencil line where the spots are, it can dissolve the compounds in the mixture, causing them to spread out and overlap. This can lead to inaccurate results and difficulties in analyzing the separated components on the chromatogram. It's important to maintain the separation of the compounds on the chromatography plate for clear identification.


What happens if the eluent is above the 1.5cm line in a chromatography experiment?

If the eluent is above the 1.5cm line in a chromatography experiment there will not be a proper distribution in a test tube to discover the sources of ink on a paper. A chromatography experiment tests for the sources of ink whether it be chemical or plant based.


What type of energy does a pencil have?

A pencil has potential energy due to its position above the ground. This potential energy can be converted into kinetic energy when the pencil is dropped and accelerates towards the ground.


What is the antonym for periscope?

well there really is no antonym that i know of for periscope because a periscope is an object.


You drop a pencil from your desk, which is 0.60 m above the floor?

Then, you pick it up.


What would happen if the solvent is above the level of initial sample spots?

If the solvent is above the level of the initial sample spots in a chromatography experiment, it can lead to the sample being dissolved or dispersed throughout the solvent, causing smudging of the spots and inaccurate separation of components. This can result in poor resolution and difficulty in analyzing the components present in the sample.


How do you make a pencil float in the air?

You can make a pencil float in the air by using powerful magnets. Place a magnet above and below the pencil to create a magnetic field that counteracts gravity and lifts the pencil. Be cautious when working with magnets to avoid any accidents or damage.


Is poking a hole in your waterbottle with a pencil bad?

Depends, will you drink the water? If so, maybe. If you're worried about it leaking, keep the pencil in, or poke it above the water line.


Why does the submarine float and submerge in water?

Submarines must be able to float to load and offload crew and cargo and to have repairs and maintenance. They are designed to submerge either for underwater exploration or to pass undetected by ships at the surface and planes and satellites above.


If you submerge a home pregnancy test strip into urine above the MAX line how will it change your results?

it could saturate it, so the reading is unclear. I would do the test again to be sure.