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Why must the solvent level be below the colored spots in chromatography?
Wiki User
∙ 8y ago
If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.
Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.
Wiki User
∙ 8y ago
Wiki User
∙ 10y agoYou're doing chromatography, correct? In which you put a piece of some sort of absorbent medium with a chemical you're trying to analyze on it in a solvent that will break down the chemical, and let the wicking of the solvent pull the components of the chemical you're analyzing up the medium? If you were to submerge the dye spot in the solvent, it'd dissolve into the solvent rather than separating out and you'd get no analysis.
Wiki User
∙ 11y agoif the solvent front would be above the spot of dyes, than the dyes would washed out in solvent and would not be able to move.
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Why is the mixture placed above the level of the solvent in chromatography?
If not, the mixture would just soak out into the liquid in the tank.
Why is it important that the level of the elution solvent not drop below the top of the absorbent?
Do not allow the solvent to drain below the absorbent because this will cause cracks.
What would happen if you submerge the chromatography paper above the pencil line?
Try and see: if it wouldn't be above the level of solvent, the spots will run off.
Why is it important to stop the chromatograph before the solvent reaches the top of the paper?
if it wouldn't be above the level of solvent the spots will run off
Why must the level ofthe solvent not reach the pencil line where the spots are?
if it wouldn't be above the level of solvent the spots will run off
Why is the mixture placed above the level of the solvent in chromatography?
If not, the mixture would just soak out into the liquid in the tank.
What would happen if the Spot of origin is lowered into the solvent in chromatography?
If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent. hope this helps. good luck in o. chem!
Why is it important that the level of the elution solvent not drop below the top of the absorbent?
Do not allow the solvent to drain below the absorbent because this will cause cracks.
What would happen if you submerge the chromatography paper above the pencil line?
Try and see: if it wouldn't be above the level of solvent, the spots will run off.
Uses for paper chromatography?
They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.
Why is it important that the sample spots are not below the solvent level when the filter paper is first placed in the developing chamber?
By placing the spot above the level of the solvent in the beaker, you slow the flow of solvent which results in improved separation of the components in the spot. If you placed it below the level of the solvent in the beaker, the solvent would just flow at a much faster rate and instead of separating the components in the spot, they would simply be washed away.
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
Why is it important to stop the chromatograph before the solvent reaches the top of the paper?
if it wouldn't be above the level of solvent the spots will run off
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Why must the level ofthe solvent not reach the pencil line where the spots are?
if it wouldn't be above the level of solvent the spots will run off
Why do you use a solvent in chromatography?
A solute will dissolve in a solvent when the attractive intermolecular forces between the molecules of the solvent and the molecules of the solute are greater than the attractive forces between one solute molecule and another. Thus the solute is effectively 'pulled apart' (on a molecular level at least) by the solvent and it's molecules become 'suspended' between the molecules of the solvent at which point the solute is said to be in solution or dissolved.
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