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Why is it important that the level of the elution solvent not drop below the top of the absorbent?
Wiki User
∙ 13y ago
Do not allow the solvent to drain below the absorbent because this will cause cracks.
Wiki User
∙ 13y ago
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Why must the solvent level be below the colored spots in chromatography?
If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.
How does change in pH in elution buffer helps elute protein from ion exchange column?
Binding to a cation or anion exchange column requires a binding buffer that is below or above the pI of the protein (respectively) and therefore an appropriate protein ionization state for binding. In a practical sense, this means that if the pI of your protein is 7.0, you would need to below this (6.5 or below) in order to bind to a cation exchange column. Changing the pH of the elution buffer will change the ionization state of the protein and therefore exchange cations.
Why is it important to stop the chromatograph before the solvent reaches the top of the paper?
if it wouldn't be above the level of solvent the spots will run off
What is a substance that other materials dissolve in?
A solvent can be described in exactly that way. The other materials here are called solutes. Solvents can be liquids or gases, and they can dissolve solids, liquids or gases to create a solution. Need a link? Look below.
Why is there a solvent in permanent markers?
The solvent is really the key to permanent markers; without this liquid carrier to dissolve and transport ink colorant and ink resin through the sponge via siphoning, markers would not work. Read more at the link below.
Why is it important that the sample spots are not below the solvent level when the filter paper is first placed in the developing chamber?
By placing the spot above the level of the solvent in the beaker, you slow the flow of solvent which results in improved separation of the components in the spot. If you placed it below the level of the solvent in the beaker, the solvent would just flow at a much faster rate and instead of separating the components in the spot, they would simply be washed away.
Why must the solvent level be below the colored spots in chromatography?
If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.
How does change in pH in elution buffer helps elute protein from ion exchange column?
Binding to a cation or anion exchange column requires a binding buffer that is below or above the pI of the protein (respectively) and therefore an appropriate protein ionization state for binding. In a practical sense, this means that if the pI of your protein is 7.0, you would need to below this (6.5 or below) in order to bind to a cation exchange column. Changing the pH of the elution buffer will change the ionization state of the protein and therefore exchange cations.
How do you dissolve polymer?
Each polymer has a specific solvent; see the link below.
In which solvent hydroxypropyl cellulose is completely soluble?
with water below 39 degree Celsius.
Why is it important to stop the chromatograph before the solvent reaches the top of the paper?
if it wouldn't be above the level of solvent the spots will run off
Solutes decrease the boiling point of a solvent?
No: the boiling point is increased; see the link below.
When is an unlisted liquid flammable solvent classified as HW?
Flash point of 21 C or below
What would happen if the Spot of origin is lowered into the solvent in chromatography?
If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent. hope this helps. good luck in o. chem!
Why do solvent particles flow into the cell when the initial volume is below 50 percent?
I believe it is all to do with diffusion.The solvent particles will move from an area of high concentration to the area of low concentration (the cell).
What is a solvent Citizen mean?
A person who lives below his means , has no debt and has easy access to more wealth than he needs .
What is a substance that other materials dissolve in?
A solvent can be described in exactly that way. The other materials here are called solutes. Solvents can be liquids or gases, and they can dissolve solids, liquids or gases to create a solution. Need a link? Look below.
