0
What would happen if the Spot of origin is lowered into the solvent in chromatography?
Wiki User
∙ 14y ago
If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent.
hope this helps. good luck in o. chem!
Wiki User
∙ 14y ago
Add your answer:
Earn +20 pts
What is propelling force in paper chromatography?
The force act to shift the component of the mixture from their point of origin and displaces them in the direction of solvent flow.
What does a small Rf number tell you about the characteristics of the moving molecules along the chromatography paper?
The retention factor is a ratio used in columnar chromatography. A small RF number signifies that the molecule crossed a small distance from the origin of applied pigments.
What would be the consequence of having so much solvent in the developing chamber that it would cover the application?
If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed. These photos show how the yellow compound is running into the solvent when lifted from the developing jar.
What does solvent mean in biology?
1_A solvent is either a liquid or gas that takes into itself a solute (which can be in the state of a solid, liquid or gas) and creates a solution.If we use a simple and easy example, we can get a handle on the idea. Take a glass of warm water, put a teaspoon of table salt in it, and stir it. The salt will dissolve in the water and "disappear" from view. The water is the solvent here, the salt is the solute in this example, and the resulting salt water is a solution that we created. It's that simple.Wikipedia has more information, and a link is provided to their post on solvent.A solvent is also the substance that dissolves the solute.2_The solvent is the substance that the solute or solutes dissolve (disappear in solvent) in and it forms the bulk of the solution . In a suger solution, sugar is the solute and water is the solvent .
Why do chromatography?
Chromatography is the methods used to separate complex mixtures. The components to be separated are distributed between two phases, a stationary and mobile phase. There are many types of chromatography: Liquid Chromatography, Gas Chromatography, Thin-layer Chromatography, and Paper Chromatography. As the separation occurs, a mixture is separated into its components. As a result, the molecules of the components have different masses, and so they travel along a medium at different rates. The process of chromatography can be used on a T-shirt. The mobile phase would be the alcohol and the stationary phase is the permanent markers. The solvent is the alcohol and the solutes are the marks made by the permanent markers. When the solvent, in this case, the alcohol, is distributed onto the shirt, some of the marks dissolve in the solvent. After a period of time, the marks on the medium, the T-shirt, end up spread out between the original spot and the point the solvent reaches. In designing the shirt, the alcohol spread the marker making concentric rings of different colors. This effect of concentric rings occurs since the different components of the permanents markers travel at different rates. In any chemical or bio-processing industry, the need to separate and purify a product from a complex mixture is a necessary and important step in the production line. For example, pharmaceutical industry uses chromatography to isolate penicillin and other antibiotics. Proteins can even be separated into amino acids through the process of chromatography. Chromatography is also used in crime scene investigation for DNA and RNA sequencing as well as in many scientific studies to identify unknown organic and inorganic compounds. This separation of mixtures is useful to us in various ways.
What is propelling force in paper chromatography?
The force act to shift the component of the mixture from their point of origin and displaces them in the direction of solvent flow.
Why are Rf values always less than 1?
Rf values are distance of substance from origin divided by distance of solvent front from origin. As the substance travels with the solvent, the solvent will always have a greater or equal value to the distance travelled by substance. This means the highest value of Rf is 1.
What does a small Rf number tell you about the characteristics of the moving molecules along the chromatography paper?
The retention factor is a ratio used in columnar chromatography. A small RF number signifies that the molecule crossed a small distance from the origin of applied pigments.
Why is it important to stop the chromatograph before the solvent reaches the top of the paper?
if it wouldn't be above the level of solvent the spots will run off
When did Ys Origin happen?
Ys Origin happened in 2006.
What is the definition of a retention factor?
The retention factor of a particular material is the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin.
What would be the consequence of having so much solvent in the developing chamber that it would cover the application?
If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed. These photos show how the yellow compound is running into the solvent when lifted from the developing jar.
What is the Origin of breaking a wishbone?
It means that you want something good to happen to you
What is the origin of the idiom 'lower the boom'?
'Lower the boom' - to scold or punish strictly Origin: A boom is a long pole used on ships. Booms are also used backstage in theatres to move scenery. If someone actually lowered a boom upon your head, you would be knocked out!
Is water the gift of life?
That is because water is a universal solvent and essential for living. Its believed that all life form evolved from water, so water is the origin of organisms existence.
Why is water the gift of life?
That is because water is a universal solvent and essential for living. Its believed that all life form evolved from water, so water is the origin of organisms existence.
How do you get the of man out of the way to get to cave of origin in emerald?
Anger the ancient Pokemon and cause craziness to happen to the weather.
