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What causes a stain to adhere to bacteria cells and why all colored dyes not necessarily useful for simple staining?
Wiki User
∙ 10y ago
The causes a stain adheres to bacterial cells is the color-bearing ions (chromophores) and they might be positively charged (cationic), such as methylene blue, basic fuchsin, and crystal violet, because bacteria are negatively charged and anionic chromophores, such as eosin, will not stain bacteria because of the electrostatic repelling forces that are involved.
Wiki User
∙ 10y ago
Wiki User
∙ 13y agoAcidic dyes contain anionic chromospores. Anionic chromospores such as eosinate- will not stain because of electrostatic repelling forces that are involved.
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Which cell structure has a role in the initiation of disease?
Fimbriae. they can help bacteria adhere
Function of heat-fix in gram staining?
Heat fixing is done to kill the specimen; adhere the specimen to the slide; and alter the specimen so that they more readily accept stains.
What do bacteria possess to allow them to adhere to intestinal lining?
Both bacteria and the lining cells of the intestinal tract have to have mutual attractive proteins. These will allow the bacteria affect the other cells. Many do not and they have no bad effect. We do have billions of bacteria that are beneficial and not harmful. The digestive tract is their normal habitat.
Of what value is a capsule to a bacterium?
A capsule to a bacterium helps bacteria adhere to surfaces and resist flushing. Several bacteria that have capsules are klebsiella, pneumoniae, bacillus subtillus, and streptococcus pneumoniae.
What are bacterial pili?
Pili are tiny, hollow projections that are used in attachment to surfaces. They have nothing to do with motility. There are two types 1.) long conjugation pili, that aide in transfer of genetic material. 2.)Attachmetn pili or fimbriae, that help bacteria adhere to surfaces. Hope that helps!!!
Purpose of fixing the bacterial film?
The purpose of heat fixing the bacteria to the slide is so during the gram staining procedure the bacteria doesn't wash off. If you didn't heat fix the bacteria to the slide, it would not stay on the slide.
What is the purpose of heat fixing the smears?
It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without serious distortion. They adhere better to the slide and also take up dye more easily.
What is the importance of heat fixing the slide before staining?
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
What cover the outside of all prokaryotes?
Along the surface of some bacteria are structures called pili that help bacteria adhere to surfaces
What the relationship between capsules and the pathogenicity of bacteria?
The presence of capsules increase the pathogenicity of the bacteria. The value of a capsule to a bacterium helps the bacteria adhere to the surfaces and resist flushing.
Which cell structure has a role in the initiation of disease?
Fimbriae. they can help bacteria adhere
Function of heat-fix in gram staining?
Heat fixing is done to kill the specimen; adhere the specimen to the slide; and alter the specimen so that they more readily accept stains.
Is presence of grease or dirt on a glass slide going to result in poor smear preparation?
The smear will not adhere well if there is grease on the slide.
Why do you need to stain bacteria?
Bacteria is stained to identify the organism and its structure. Staining also can help in identification of a species. Bacteria are translucent in appearance therefore a stain is applied to allow them to become visible.
What is an adhesin?
An adhesin is any of various factors which enables bacteria to adhere to epithelial surfaces as a step towards infection.
What do pili adhere to bacteria for and what is this function called?
pilli is used for adhesion to substatum and some sex pilli are used in reproduction
