copper(II) ions + protein (2 or more peptide bonds) ---> biuret complex (PURPLE!)
the intensity of the purple depends on how many copies of the complex have been formed.
biuret reagent chemical formula is comprised of potassium hydroxide and copper sulfate
The chemical formula for biuret is H2NC(O)NHC(O)NH2
10% NaOH
0.5% CuSO4
egg albumin
Biuret reagent is used to test for protein in urine. It is a common test that students in biology class perform. Urine is added to a test tube, followed by approximately the same amount of Biuret reagent. If the solution turns lavender this means that there are proteins present in the urine.
yes
Amino acids doesn't react with the biuret reagent.
Benedicts reagent is used to check reducing sugars , it need hat to work and gives red color when positive ,. While biuret reagent is for proteins .there is no need to use heat and gives purple , violet color when positive ..
Use Biuret reagent.
Biuret reagent detect peptide bonds.
Buiret Reagent
Biuret reagent is used to test for protein in urine. It is a common test that students in biology class perform. Urine is added to a test tube, followed by approximately the same amount of Biuret reagent. If the solution turns lavender this means that there are proteins present in the urine.
biuret reagent
yes
Biuret reagent test in a practical situation tests for lipids. Biuret is used in food agents for protein amino acid chains.
Amino acids doesn't react with the biuret reagent.
The Biuret reagent can be used.
Benedicts reagent is used to check reducing sugars , it need hat to work and gives red color when positive ,. While biuret reagent is for proteins .there is no need to use heat and gives purple , violet color when positive ..
The biuret reagent should not react with a single amino acid. The reagent reacts when there is a peptide bond linking amino acids together. If you are seeing the biuret reagent react in the presence of a single amino acid, then there must be some amino acids that are still linked together.
Biuret reagent react with the skin proteins.
Purple or black