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What might Biuret reagent test in a practical real life situation?
Wiki User
∙ 9y ago
Biuret reagent test in a practical situation tests for lipids. Biuret is used in food agents for protein amino acid chains.
Wiki User
∙ 9y ago
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Where can you get biuret solution?
Found this place in my own personal quest for some. There might be better. http://www.sciencecompany.com/sci-exper/food_chemistry.htm#Biuret%20Reagent
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Situation where you might use the Biuret Reagent colorimetric test?
To test for the presence of starch in cells.
Where can you get biuret solution?
Found this place in my own personal quest for some. There might be better. http://www.sciencecompany.com/sci-exper/food_chemistry.htm#Biuret%20Reagent
What is the advantages of using biuret reaction in place of measuring the absorption of the protein solution at 280 nm?
The absorption of proteins at 280nm is according to electrons from the rings on the amino acid such as His, Trp, etc. And if there is no such kind of amino acids in the protein, we might not be able to get what the amount of the protein really is. At the other hand, what makes biuret reaction work is by the copper ion reacting with the dipeptide bonds, since every amino acid has the peptide bond, it's more accurate and reasonable to use biuret reactions to determine what the amount of the protein is.
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