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Lac+ bacteria is acid base color indicator that lowers the pH of the MacConkey agar. The MacConkey agar contains neutral red dye, lactose, peptone, and crystal violet dye.
MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.
this MacConkey with crystal violet can grow enterococcus
MacConkey agar contains bile salts, lactose, peptone. crystal violet dye, and neutral red dye. The agar appears a medium pink with a copper undertone.
Violet Red Bile Agar is recommended for use in the microbiological analysis of milk and other dairy products and for use in the examination of water. Violet Red Bile Agar is a selective medium which detects the growth of lactose fermenting coliforms. Coliforms colonies lower the pH of the medium subsequently causing their colonies to look red (neutral red dye) and to precipitate the bile salts. Crystal violet and bile salts inhibit the growth of Gram positive microorganisms.
Staphylococcus will not grow on Mac since crystal violet inhibits the growth of Gram positivesfmchinea: Actually some species of staphylococcus grow on MacConkey's plate including Staphylococcus aureus.
its because
ecoli is a lactose metabolizer, can live on macconkey while psuedomoas cannot.
It would allow growth of Gram-positive cocci.
crystal violet, tellurite, and trypan blue. it's used to isolate streptococcus/enterococcus strains (they absorb the trypan blue)
Well, Macconkey Agar II plates have Gram Crystal Violet in them so there are a number of bacterial species that can absorb it and turn "very pink." The way to narrow it down is to identify whether it grows in chains or clusters. But other than that, it is hard to tell.
E.coli shows pink colour colonies in MacConkey agar due to fermentation of lactose