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He wondered if testing for two separate characteristics would cause them to somehow interact. This law only applies when two traits are tested like seed color and texture.

Traits are independent ONLY if they occur on separate chromosomes. Lucky for Mendel that the 7 traits he tested all occurred on 7 separate chromosomes.

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Q: What did Mendel wonder about that caused him to establish his law of independent assortment?
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What states that each gene pair is inherited independently of the gene pairs for other traits?

This law is called Mendel's second law, or the Law of Independent Assortment. The law is derived from observations of dihybrid crosses. A classic example involves seed shape and color in garden peas. The shape may be round (caused by a dominant allele, denoted by R) or wrinkled (recessive, r). The wrinkling is caused by a mutation preventing the formation of branches in starch molecules. The color of the seeds may be yellow (dominant, Y) or green (recessive, y). The green color results from a variant sequence in a gene; the seeds fail to develop normally. Parentals are RRYY and rryy. The F1 plants are RrYy. The F2 plants show independent segregation of the alleles for the two characteristics, shape and color. A Punnett grid predicts a 9:3:3:1 ratio for the phenotypes round yellow, round green, wrinkled yellow, and wrinkled green.


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