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They prevent unspecific interactions between proteins and the membrane, thus reducing background.
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∙ 15y ago
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What reagent is used to test for starch?
The reagent that is used to test for starch is a mixture of iodine and potassium iodide in water, or an Iodine - KI reagent. If the reagent turns blue-black in color, then starch is present.
What is the reagent used to test proteins?
Buiret Reagent
Why BSA is used as standard for protein estimation?
the answer is that BSA is most common protein available as it is cheap and alsocontains tyrosine and tryptophan as amino acids which are important for breaking the peptide bonds
What color is million's reagent?
blue
What is million reagent?
You probably mean Millon's reagent. Millon's reagent is a solution of mercury in nitric acid which is used as a test for the amino acid tyrosine. Since proteins contain tyrosine, it is also used as a test for protein. When the test solution is boiled with Millon's reagent a white precipitate (solid) is produced which coagulates and turns red.
How can a tween be spontaneous?
A tween can be spontaneous by being themselves.
Is 9-12 a tween?
10 - 12 Is a Tween :)
Can you use tween 80 as a substitute of tween 20?
I have tried tween 80 for western blotting. It seems to work the same as tween 20 (I can't tell the difference).
What is tollen's reagent and baeyer's reagent?
tollen's reagent = [Ag(NO)2]+ baeyer's reagent= KMnO4
What is bsa solution?
BSA solution is Bovine Serum Albumin
When was BSA Company created?
BSA Company was created in 1978.
Equation of formaldehyde schiff's reagent benzaldehyde schiff's reagent and acetone schiff's reagent?
Formaldehyde schiffs reagent Formaldehyde schiffs reagent
When was Tween Brands created?
Tween Brands was created in 1987.
When did Tween Brands end?
Tween Brands ended in 2009.
What is classic tween?
tween is me im a tween and im original out there totally weird and its awesome im 11 and cant fit most pant cause im tall and thin and everything is either to grown up or to baby tween is me im a tween
Why milk as a blocking reagent?
Most people use it because it's cheap. A blocking reagent is used to saturate any binding sites on your membrane/plate/whatever that will bind any old bit of protein just because it's protein, not because it's the thing you're looking for. Milk (or skimmed milk powder, which is what most people use) is just a big old load of protein. You'll find people will use BSA (bovine serum albumin) or other types of serum as blocks in other protocols. It all boils down to the same thing. Big old lot of protein that saturates all your non-specific binding sites before you start probing for your protein of interest. Means you should get a nice clean signal instead of a mucky one.
When you are 13 does that mean you're still a tween or not?
When you are 13 you are still a tween, nothing has changed your not suddenly a teenager so definatley tween!
