butt colour and reaction - yellow slant colour and reaction - yellow carbohydrate fermented - glucose only H2S production :- blackening- NO H2S - (-) negative
Gram Stain to determine gram negative and shape or streak on a Agar plate selective for gram negative such as Desoxycholate Agar (DES). A SIM (Sulfur, Indole, Motility) Test or a Kiliger's Iron Agar (KIA) Test can confirm the production of H2S. *I would choose Streaking on DES to isolate the gram Negative bacteria, and then stab inoculate a SIM tube. Black precipitate confirms H2S production positive.
You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.
The formula for dihydrogen sulfide, or just hydrogen sulfide, is H2S. A pair of hydrogen atoms are bonded to a sulfur atom to make up this toxic compound. A link can be found below for more information.
It is known as hydrosulfuric acid. Not to be confused with sulfuric acid which is H2SO4
why the H2S production test would be diagnostically significant?
TioAbsorb the H2S Gas produced during the test raction.
H2s
H2S 63.1 g H2S * 1 mol H2S / 34.076 g H2S = 1.85 mol H2S
butt colour and reaction - yellow slant colour and reaction - yellow carbohydrate fermented - glucose only H2S production :- blackening- NO H2S - (-) negative
Gram Stain to determine gram negative and shape or streak on a Agar plate selective for gram negative such as Desoxycholate Agar (DES). A SIM (Sulfur, Indole, Motility) Test or a Kiliger's Iron Agar (KIA) Test can confirm the production of H2S. *I would choose Streaking on DES to isolate the gram Negative bacteria, and then stab inoculate a SIM tube. Black precipitate confirms H2S production positive.
The formula for dihydrogen monosulfide is H2S.
You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.
H2S = hydrogen sulphide
No, H2S is hydrosulfuric acid. Sulfuric acid is H2SO4
The formula for dihydrogen sulfide, or just hydrogen sulfide, is H2S. A pair of hydrogen atoms are bonded to a sulfur atom to make up this toxic compound. A link can be found below for more information.
H2S is a polar compound.It is not ionic.