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Gram Stain to determine gram negative and shape or streak on a Agar plate selective for gram negative such as Desoxycholate Agar (DES). A SIM (Sulfur, Indole, Motility) Test or a Kiliger's Iron Agar (Kia) Test can confirm the production of H2S. *I would choose Streaking on DES to isolate the gram Negative bacteria, and then stab inoculate a SIM tube. Black precipitate confirms H2S production positive.

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Q: What is the best biochemical test to identify gram negative and H2S bacilli?
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