size and shape
Because it is a Gram-negative bacterium and can be stained with Gram-negative stain.
negative staining is when the micro-organism on slide is not stained.. instead the rest of the material on the slide gets stained and the organism stands out prominently unstained.. this occurs because that organism has a thick capsule around it which is non-stainable with that stain.. example is pneumococci staining with India ink.
Negative staining has a dark contrasted background and the bacteria is white. Simple staining has a white background and bacteria is the color depended on your stain color.Negative staining when prepared is NOT heat fixed but simple staining when prepared is heat fixed. Heat fixed means when preparing slide with bacteria on it, it is passed over some type of flame, like a Bunsen burner flame, three times or four times.
Not all oral bacteria are gram negative, but those that are there can be seen with Gram stain.
The negative staining techniques uses a dye solution in which the chromogen is acidic and carries a negative charge. (An acidic chromogen gives up a hydrogen ion, which leaves it with a negative charge.) The negative charge on the bacterial surface repels the negatively charged chromogen, so the the cell remains unstained against a colored background.
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
Capsule Stain
Because it is a Gram-negative bacterium and can be stained with Gram-negative stain.
Bacteria .
Fontana silver staining.
Because the cell wall repels the binding of the negative stain therefore the cells do not stain. Because of this the background is stain with the dye used and the bacteria remain colorless. Basically your staining the background, that is, you are not directly staining the cells.
Those which show pink colour in gram staining are gram negative bacterialike E.coli
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
negative staining is when the micro-organism on slide is not stained.. instead the rest of the material on the slide gets stained and the organism stands out prominently unstained.. this occurs because that organism has a thick capsule around it which is non-stainable with that stain.. example is pneumococci staining with India ink.
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Gram Negative