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If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
Is the enzyme that allows replication to occur DNA polymerase or RNA polymerase?
DNA polymerase replicated DNA. RNA polymerase creates mRNA to be used in protein synthesis. RNA polymerase does not replicated DNA.
Kary Mullis discovered a type of DNA polymerase that could withstand high temperatures Why did scientists want to heat up DNA replication?
This is because of Polymerase Chain Reaction (PCR). Basically, the problem is that you have a mixture of DNA, polymerase, primers etc, and you want to denature the DNA (separate both chains) - the denaturation happens at 94°C. Since the polymerase is present in the mixture, it has to withstand such temperature.
Is DNA polymerase a protein?
Yes, DNA polymerase is a protein.
Role of Taq polymerase in PCR?
taq polymerase is special because it is very stable at high temperatures and will not denature even at the 90 degree step of pcr. taq polymerase is so heat stable because it was extracted from the bacterium thermus aquaticus, which is found in hot springs and geezers
What enzyme transcribes DNA?
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
What are the key differences between DNA polymerase and RNA polymerase in terms of their functions and roles in the process of genetic information replication?
DNA polymerase is responsible for synthesizing new DNA strands during DNA replication, while RNA polymerase is responsible for transcribing DNA into RNA. DNA polymerase adds nucleotides to the growing DNA strand, ensuring accurate replication of genetic information. RNA polymerase reads the DNA template and synthesizes a complementary RNA strand. Overall, DNA polymerase is involved in DNA replication, while RNA polymerase is involved in transcription.
What is the name of the enzyme that match the DNA bases?
DNA polymerase matches the bases on the parent strand.
How does DNA polymerase function in the process of transcription?
DNA polymerase does not function in the process of transcription. Transcription is the process where RNA is synthesized from a DNA template by RNA polymerase. DNA polymerase, on the other hand, is involved in DNA replication, where it synthesizes a new DNA strand using a DNA template.
What is the name of the two enzymes that help in the duplication of DNA?
More than two enzymes are involved. However, the main ones are DNA Polymerase I and DNA Polymerase III. DNA Polymerase III adds new nucleotides and DNA Polymerase I removes primers.
Is DNA polymerase used in the process of transcription?
No, DNA polymerase is not used in the process of transcription. Transcription is the process of making an RNA copy of a gene from DNA, and it is carried out by an enzyme called RNA polymerase. DNA polymerase is primarily involved in the process of DNA replication.
