Agar, in agar plates used in culturing bacteria and other microorganisms, consists of a gelling agent, and is an unbranched polysaccharide obtained from the cell walls of some species of red algae, primarily from the genera Gelidium and Gracilaria, or seaweed. As very few bacteria are able to decompose the actual agar itself, nutrients such as glucose must be added to the agar mixture to allow bacterial growth. It is these various nutrients that the bacteria feed on to survive.
The typical agar plate contains:
# A carbon source such as glucose for bacterial growth # Water # Agar # Various salts needed for bacterial growth # A source of amino acids and nitrogen (e.g., beef, yeast extract) This particular mix is an undefined medium because the amino acid source contains a variety of compounds with the exact composition being unknown. In this case, most bacteria will be using all the above except 3. Agar for nutrition.
Bacteria uses peptones for nutrients in a nutrient agar. Peptones are a soluble form of proteins that are formed in the early stage of protein breakdown, during digestion.
that they are resistant to ampicillin, they contain recombinant DNA, and they contain a human gene.
Any bacteria with right nutrient in vitro can be cultured. This method is very useful when carrying out any experiment. As long as nutrient medium is maintained bacteria will grow with no problems in a petri dish.
Inoculation is the process were in you will introduce bacterial samples on a nutrient broth or nutrient agar. If the medium you use contain bacteria it is now called inoculum. there are different technique in introducing bacteria in the medium like streaking, spreading, pouring.
They are grown on medium that contains the nutrient that they cannot synthesize
Milk contains natural sugars which can feed bacteria. It can also contain bacteria due to improper storage or handling. Water that is running swiftly over rocks can defeat bacteria in it. Stagnant water is most likely to contain bacteria.
that they are resistant to ampicillin, they contain recombinant DNA, and they contain a human gene.
Any bacteria with right nutrient in vitro can be cultured. This method is very useful when carrying out any experiment. As long as nutrient medium is maintained bacteria will grow with no problems in a petri dish.
Inoculation is the process were in you will introduce bacterial samples on a nutrient broth or nutrient agar. If the medium you use contain bacteria it is now called inoculum. there are different technique in introducing bacteria in the medium like streaking, spreading, pouring.
They are grown on medium that contains the nutrient that they cannot synthesize
Complex culture media is a type of nutrient for growing bacteria that does not have exact composition. For example it may contain beef broth which has many nutritional components but the exact ratio of each component is not known. The opposite would be defined medium which contains exact amount of specific nutrients.
A nutrient broth is contains substances that are required for the growth of microorganisms such as bacteria. The bacteria obtain their minerals and vitamins from peptone, meat and yeast extract that comprise the broth.
Bacteria will grow in blood but no the growing medium in petri dishes should be clear agar.
Assume you are growing bacteria on a lipid medium that started at pH 7. The action of bacterial lipases should cause the pH of the medium to increase or decrease? Why?
Hydroponics (From the Greek hydro, water, and ponics, labour) is a method of growing plants using mineral nutrient solutions, in water, without soil. Terrestrial plants may be grown with their roots in the mineral nutrient solution only or in an inert medium, such as perlite, gravel, mineral wool, or coconut husk.
the bacteria comes in contact with nurturing, growing medium like mucus or saliva
The purpose of the thio-glycolate broth, which, if memory serves, is a semi-solid agar, is to determine oxygen requirements. E. coli is a facultative anaerobe which means it prefers aerobic conditions but can grow (to a lesser extent) anaerobically. So you will see growth at the top of the thio-glycolate tube mainly where there is oxygen, perhaps some just along the shallow stab line from inoculation. It will not permeate throughout the medium since it prefers aerobic respiration and oxygen is available at the top.
Milk contains natural sugars which can feed bacteria. It can also contain bacteria due to improper storage or handling. Water that is running swiftly over rocks can defeat bacteria in it. Stagnant water is most likely to contain bacteria.