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Why does E-coli grow in nutrient broth better than in thio-glycolate broth?
Wiki User
∙ 14y ago
The purpose of the thio-glycolate broth, which, if memory serves, is a semi-solid agar, is to determine oxygen requirements. E. coli is a facultative anaerobe which means it prefers aerobic conditions but can grow (to a lesser extent) anaerobically. So you will see growth at the top of the thio-glycolate tube mainly where there is oxygen, perhaps some just along the shallow stab line from inoculation. It will not permeate throughout the medium since it prefers aerobic respiration and oxygen is available at the top.
Wiki User
∙ 14y ago
Wiki User
∙ 9y agoChemically defined media does not contain any buffer thus can't maintain the suitable pH for the growing of bacteria because it can't neutralised the metabolic waste. The nutrient broth medium contain natural buffer like amino acid and peptone that can neutralise the metaboic waste of E. coli thus allowing the bacteria to keep growing.
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Why is thioglycolate broth stored at room temperature?
There is a decreased absoportion of oxygen by the mediium.
Why is nutrient broth better than peptone broth?
Nutrient broth contains beef extract which acts as sources of amino acids, peptides, nucleotides, organic acids, vitamins and mineralsthat peptone broth don't contain? :)
What would be a nitrogen source for the nutrient broth or agar?
the east extract is the main source of nitrogen in the nutrient broth or agar
Does Bacillus subtilis grow in MacConkey broth?
Bacillus subtilis is grown on nutrient agar or in nutrient broth. The preferred medium is M9 with glucose.
What are the essential ingredients of a carbohydrate broth?
nutrient broth + 0.5% particular carbohydrate + indicator
Is it necessary to cook the nutrient broth?
To destroy any competitors for the broth, fungi and bacteria.
Is nutrient broth suitable for the growth of fungal species?
NO
Which technique agar or broth provides more information?
Out of the two, nutrient agar will provide more information on the variety of bacteria that are in an environment. Nutrient agar is a better environment for bacteria and fungi to thrive in.
Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow where?
the ampicillin broth and the nutrient broth
Use of sodium chloride in nutrient broth?
Nutrient broth is a liquid used to grow bacterial cultures and the amount of sodium chloride is a solution of 0.90% w/v of NaCl.
Why does nutrient broth go cloudy?
Nutrient broth becoming cloudy in appearance is usually a good indication that one or more cultures of bacteria or fungi has grown in the broth. This can be confirmed by viewing a sample of the broth under a simple light microscope, or by streaking a sample onto nutrient agar and incubating it to see if colonies become visible (usually within a few days).
When you examined your incubated nutrient broth tube you compared it to an uninoculated broth tube The uninoculated tube was a control What is control?
no
