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The highest note on a guitar is on the last fret of the high E string.

Different Guitars have different number of frets, hence the highest note varies depending on the instrument.

On vintage fender guitars, such as stratocasters from the 1950's there were only 21 frets, hence the highest note was a Db

Most guitars today have 22 frets, making the highest note a D, but there is an increasingly large number of 24 fret guitars from brands such as ibanez, schecter and esp that push the highest note to E, giving the guitar a full range of 4 octaves.

In recent years, ibanez manufactured the 27 fret Xiphos, which make the highest note F#

Of course there are many, techniques such as pinch harmonics, and effect pedals which allow the guitar to reach tonalities beyond that of the last fret, but as far as normal notes are concerned the ones shown are the highest, given the guitar is in standard tuning

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